Zijlstra C, Schärer-Hernández N, Gal S, Hohn T
Friedrich Miescher-Institut, Basel, Switzerland.
Virus Genes. 1996;13(1):5-17. doi: 10.1007/BF00576974.
Expression of open reading frame (ORF) VI of cauliflower mosaic virus (CaMV) in transgenic Arabidopsis thaliana caused a typical syndrome characterised by leaf chlorosis, vein clearing, plant stunting and reduced fertility. In addition and in comparison to untransformed controls we observed the formation of much larger rosettes of leaves combined with much later flowering and more extensive tillering. In these aspects, the ORF VI transgenic plants resembled late flowering mutants. All these phenotypes correlated with expression of ORF VI in three lines of transgenic plants which were produced independently, with different Ti-plasmid derived vectors and with different selective markers. The late flowering phenotype cosegregated with the transgene.
花椰菜花叶病毒（CaMV）开放阅读框（ORF）VI在转基因拟南芥中的表达导致了一种典型的综合征，其特征为叶片黄化、叶脉清晰、植株矮小和育性降低。此外，与未转化的对照相比，我们观察到转基因植株形成了更大的莲座叶丛，开花时间更晚，分蘖更广泛。在这些方面，ORF VI转基因植株类似于晚花突变体。所有这些表型都与独立产生的三株转基因植株中ORF VI的表达相关，这些植株使用了不同的源自Ti质粒的载体和不同的选择标记。晚花表型与转基因共分离。
