花椰菜花叶病毒感染的芜菁原生质体中病毒基因产物的积累动力学

Accumulation kinetics of viral gene products in cauliflower mosaic virus-infected turnip protoplasts.

作者信息

Kobayashi K, Nakayashiki H, Tsuge S, Mise K, Furusawa I

机构信息

Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Japan.

出版信息

Microbiol Immunol. 1998;42(1):65-9. doi: 10.1111/j.1348-0421.1998.tb01972.x.

DOI:10.1111/j.1348-0421.1998.tb01972.x

PMID:9525783

Abstract

The expression of cauliflower mosaic virus (CaMV) genes was studied in a turnip protoplast system. Six CaMV-encoded gene products were detected in infected turnip protoplasts by means of Western blotting. The infected turnip protoplasts showed different patterns of protein accumulation; e.g. an open reading frame (ORF) I-encoded movement protein, an ORF V-encoded reverse transcriptase and an ORF VI-encoded posttranscriptional transactivator representing the early accumulated proteins, an ORF II-encoded aphid transmission factor and an ORF IV-encoded coat protein the late accumulated proteins and an ORF III-encoded DNA binding protein the intermediate protein. The results suggest that the expression of CaMV genes is differentially regulated.

摘要

在芜菁原生质体系统中研究了花椰菜花叶病毒（CaMV）基因的表达。通过蛋白质免疫印迹法在受感染的芜菁原生质体中检测到六种CaMV编码的基因产物。受感染的芜菁原生质体表现出不同的蛋白质积累模式；例如，一个开放阅读框（ORF）I编码的运动蛋白、一个ORF V编码的逆转录酶和一个ORF VI编码的转录后反式激活因子代表早期积累的蛋白质，一个ORF II编码的蚜虫传播因子和一个ORF IV编码的外壳蛋白是晚期积累的蛋白质，一个ORF III编码的DNA结合蛋白是中期蛋白质。结果表明，CaMV基因的表达受到差异调节。