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通过荧光原位杂交对黑麦草属物种核糖体DNA位点的比较。

Comparison of ribosomal DNA sites in Lolium species by fluorescence in situ hybridization.

作者信息

Thomas H M, Harper J A, Meredith M R, Morgan W G, Thomas I D, Timms E, King I P

机构信息

Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth.

出版信息

Chromosome Res. 1996 Nov;4(7):486-90. doi: 10.1007/BF02261775.

DOI:10.1007/BF02261775
PMID:8939359
Abstract

The position of the 18S-5.8S-26S and 5S rRNA genes have been physically mapped on the chromosomes of seven Lolium taxa. 18S-5.8S-26S sites were seen on two pairs of chromosomes in the inbreeding taxa. In the outbreeding taxa six sites were found in the L. multiflorum, seven in L. perenne and nine in L. rigidum var. rigidum. Two 5S sites were found in each of the taxa. In the inbreeders, the 5S sites were found adjacent to the 18S-5.8S-26S sites on chromosome 2. In L. multifiorum and L.perenne the 5S sites were on the short arm of chromosome 3. However, in L. rigidum var. rigidum the 5S rDNA site was found in either of the two positions.

摘要

18S - 5.8S - 26S和5S核糖体RNA(rRNA)基因的位置已在7个黑麦草分类群的染色体上进行了物理定位。在自交分类群的两对染色体上发现了18S - 5.8S - 26S位点。在异交分类群中,多花黑麦草发现了6个位点,多年生黑麦草发现了7个位点,硬直黑麦草变种硬直黑麦草发现了9个位点。在每个分类群中都发现了两个5S位点。在自交植物中,5S位点位于第2号染色体上与18S - 5.8S - 26S位点相邻的位置。在多花黑麦草和多年生黑麦草中,5S位点位于第3号染色体的短臂上。然而,在硬直黑麦草变种硬直黑麦草中,5S rDNA位点位于两个位置中的任意一个。

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