新闻与短讯：来自美国太平洋西北部和加拿大不列颠哥伦比亚省的毁灭盘多毛孢分离物中的双链RNA

News & Notes: Double-Stranded RNA in Isolates of Discula destructiva from the Pacific Northwestern United States and British Columbia, Canada.

作者信息

Yao JM, McElreath SD, Tainter FH

机构信息

Department of Forest Resources, Clemson University, Clemson, SC 29634, USA

出版信息

Curr Microbiol. 1997 Jan;34(1):67-9. doi: 10.1007/s002849900146.

DOI:10.1007/s002849900146

PMID:8939805

Abstract

Twenty-nine isolates of Discula destructiva Redlin from the Pacific northwestern United States and British Columbia, Canada were extracted for the presence of double-stranded RNA by use of phenol-chloroform and CF-11 chromatography. Seven/29 were positive. Agarose-gel banding profiles of the western isolates were different from those found previously in 80/80 isolates from the eastern U.S. These results suggest that isolates of D. destructiva responsible for dogwood anthracnose in Washington, Oregon, Idaho, and British Columbia may have an origin different from that of the eastern isolates.

摘要

从美国太平洋西北部和加拿大不列颠哥伦比亚省采集了29株毁灭盘多毛孢（Discula destructiva Redlin）菌株，通过苯酚 - 氯仿法和CF - 11柱层析法提取双链RNA，其中7/29呈阳性。西部菌株的琼脂糖凝胶电泳条带图谱与之前在美国东部80/80株菌株中发现的不同。这些结果表明，在华盛顿、俄勒冈、爱达荷和不列颠哥伦比亚导致山茱萸炭疽病的毁灭盘多毛孢菌株可能与东部菌株来源不同。