Rong R, Rao S, Scott S W, Tainter F H
Department of Forest Resources, Clemson University, Clemson, South Carolina 29634, USA.
Curr Microbiol. 2001 Feb;42(2):144-8. doi: 10.1007/s002843321.
DsRNAs were detected in 85/108 isolates of Discula destructiva, the cause of dogwood anthracnose, collected in South Carolina, Idaho, and Alabama. The eastern isolates contained a greater diversity of dsRNA than did Idaho isolates, but most isolates, irrespective of state of origin, contained two small bands (ca. 1.5-2.5 kb) with sequence homology indicated by Northern hybridization. Differences in the banding patterns suggest that genetic diversity of dsRNA in D. destructiva is generated rapidly and that D. destructiva can be simultaneously infected by multiple dsRNA viruses.
在从南卡罗来纳州、爱达荷州和阿拉巴马州采集的108株毁灭盘菌(山茱萸炭疽病的病原菌)分离物中,85株检测到双链RNA(dsRNA)。东部的分离物比爱达荷州的分离物含有更多样化的dsRNA,但大多数分离物,无论其来源州,都含有两条小条带(约1.5 - 2.5 kb),Northern杂交显示它们具有序列同源性。条带模式的差异表明,毁灭盘菌中dsRNA的遗传多样性迅速产生,并且毁灭盘菌可以同时被多种dsRNA病毒感染。