Caetano-Anollés G, Trigiano R N, Windham M T
Department of Ornamental Horticulture and Landscape Design, University of Tennessee, Knoxville 37901-1071, USA.
FEMS Microbiol Lett. 1996 Dec 15;145(3):377-83. doi: 10.1111/j.1574-6968.1996.tb08604.x.
Isolates of Discula destructiva Redlin and an undescribed species of Discula, the filamentous fungi that cause anthracnose of flowering (Cornus florida L.) and Pacific (Cornus nuttalli Aud.) dogwoods, were analyzed for genetic variation by nucleic acid scanning with arbitrary mini-hairpin oligonucleotide primers. While the fungal population was highly homogeneous throughout the disease range in eastern and western North America, the generation of arbitrary signatures from amplification profiles (ASAP) distinguished isolates from the northeast, middle and southern Appalachian mountain range, and western United States and Canada. ASAP involves a dual-step arbitrary primer-based amplification procedure that generates a large number of informative allelic characters by amplification of monomorphic DNA fingerprints. ASAP analyses showed a fine fungal population structure consistent with the recent and separate introduction of the pathogen on the west and east coasts of North America.
对引起花楸木（多花梾木）和太平洋梾木炭疽病的丝状真菌——毁灭盘菌（Discula destructiva Redlin）以及一种未描述的盘菌属物种的分离株，使用任意小发夹寡核苷酸引物进行核酸扫描，以分析其遗传变异。虽然在北美东部和西部的整个病害范围内，真菌种群高度同质，但通过扩增图谱生成任意特征（ASAP）可区分来自东北部、阿巴拉契亚山脉中部和南部以及美国西部和加拿大的分离株。ASAP涉及一个基于任意引物的两步扩增程序，通过扩增单态性DNA指纹产生大量信息丰富的等位基因特征。ASAP分析显示出精细的真菌种群结构，这与该病原体最近分别在北美西海岸和东海岸引入一致。
