Macalma T, Otte J, Hensler M E, Bockholt S M, Louis H A, Kalff-Suske M, Grzeschik K H, von der Ahe D, Beckerle M C
Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA.
J Biol Chem. 1996 Dec 6;271(49):31470-8. doi: 10.1074/jbc.271.49.31470.
Zyxin is a component of adhesion plaques that has been suggested to perform regulatory functions at these specialized regions of the plasma membrane. Here we describe the isolation and characterization of cDNAs encoding human and mouse zyxin. Both the human and mouse zyxin proteins display a collection of proline-rich sequences as well as three copies of the LIM domain, a zinc finger domain found in many signaling molecules. The human zyxin protein is closely related in sequence to proteins implicated in benign tumorigenesis and steroid receptor binding. Antibodies raised against human zyxin recognize an 84-kDa protein by Western immunoblot analysis. The protein is localized at focal contacts in adherent erythroleukemia cells. By Northern analysis, we show that zyxin is widely expressed in human tissues. The zyxin gene maps to human chromosome 7q32-q36.
斑联蛋白是粘着斑的一个组成部分，有人提出它在质膜的这些特殊区域发挥调节功能。在此我们描述编码人和小鼠斑联蛋白的cDNA的分离及特性。人和小鼠的斑联蛋白都有一系列富含脯氨酸的序列以及三个LIM结构域拷贝，LIM结构域是在许多信号分子中发现的一种锌指结构域。人斑联蛋白与涉及良性肿瘤发生及类固醇受体结合的蛋白质在序列上密切相关。通过蛋白质免疫印迹分析，针对人斑联蛋白产生的抗体识别出一种84 kDa的蛋白质。该蛋白质定位于贴壁的红白血病细胞中的粘着斑。通过Northern分析，我们表明斑联蛋白在人体组织中广泛表达。斑联蛋白基因定位于人类染色体7q32 - q36。
