Davis-Smyth T, Duncan R C, Zheng T, Michelotti G, Levens D
Laboratory of Pathology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Biol Chem. 1996 Dec 6;271(49):31679-87. doi: 10.1074/jbc.271.49.31679.
The cloning and expression of two new human cDNAs encoding proteins highly related to the far upstream element-binding protein (FBP) are described. FBP, FBP2, and FBP3 comprise a family of single-strand DNA- binding proteins that possess all of the general features of more conventional transcription factors. The FBPs each bind sequence specifically to only one strand of the far upstream element (FUSE; originally identified upstream of c-myc), and each possesses potent activation domains when fused to the GAL4 DNA-binding domain and assayed by transient transfection. Typical of transcription factors, the proteins are most highly related in their central, DNA-binding domains, but extensive homology is also shared within the tyrosine-rich, carboxyl-terminal activation domains. Comparison with GenBank sequences revealed a fourth FBP family member encoded by Caenorhabditis elegans chromosome III, illustrating the high degree of homology in this evolutionarily ancient and conserved family.
本文描述了两个新的人类cDNA的克隆与表达，这两个cDNA编码的蛋白质与远上游元件结合蛋白（FBP）高度相关。FBP、FBP2和FBP3构成了一个单链DNA结合蛋白家族，它们具有更为传统的转录因子的所有一般特征。每个FBP都能特异性地与远上游元件（FUSE；最初在c-myc上游被鉴定）的一条链结合，并且当与GAL4 DNA结合结构域融合并通过瞬时转染进行检测时，每个FBP都具有强大的激活结构域。作为转录因子的典型特征，这些蛋白质在其中心的DNA结合结构域中相关性最高，但在富含酪氨酸的羧基末端激活结构域中也存在广泛的同源性。与GenBank序列的比较揭示了秀丽隐杆线虫三号染色体编码的第四个FBP家族成员，这说明了这个在进化上古老且保守的家族中的高度同源性。
