Shaw R D, Hempson S J
Northport VA Medical Center, New York 11768, USA.
J Infect Dis. 1996 Dec;174(6):1328-31. doi: 10.1093/infdis/174.6.1328.
Mucosal antibody induction by otherwise identical replication-competent and replication-incompetent rhesus monkey rotaviruses was compared. Although psoralen-inactivated virus induced diarrhea, the magnitude of the intestinal antibody response was severely attenuated compared with that of replicating rotavirus, as determined by assay of mucosal antibody-secreting cells. Rotavirus that was neutralized by monoclonal antibodies (anti-VP4 and -VP7) prior to inoculation was similarly ineffective at induction of specific antibodies in intestinal secretions. In contrast to genetically inactivated virus, antibody-neutralized virus did not induce diarrhea. In this murine model, viral replication is an important determinant of antibody induction. The diarrhea response is blocked by neutralizing antibodies, but the mechanism of action is not exclusively the inhibition of viral replication.
比较了具有复制能力和无复制能力的恒河猴轮状病毒在诱导黏膜抗体方面的情况，二者在其他方面均相同。虽然补骨脂素灭活病毒会引发腹泻，但通过检测黏膜抗体分泌细胞发现，与复制型轮状病毒相比，肠道抗体反应的强度严重减弱。接种前被单克隆抗体（抗-VP4和-VP7）中和的轮状病毒在诱导肠道分泌物中特异性抗体方面同样无效。与基因灭活病毒不同，抗体中和病毒不会引发腹泻。在这个小鼠模型中，病毒复制是抗体诱导的一个重要决定因素。腹泻反应被中和抗体阻断，但其作用机制并非仅仅是抑制病毒复制。
