霍乱弧菌O139孟加拉株一个区域的克隆、测序及其在基于聚合酶链反应的检测中的应用
Cloning and sequence of a region of Vibrio cholerae O139 Bengal and its use in PCR-based detection.
作者信息
Falklind S, Stark M, Albert M J, Uhlén M, Lundeberg J, Weintraub A
机构信息
Karolinska Institute, Department of Immunology, Microbiology, Pathology and Infectious Diseases, Huddinge University Hospital, Sweden.
出版信息
J Clin Microbiol. 1996 Dec;34(12):2904-8. doi: 10.1128/jcm.34.12.2904-2908.1996.
We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region could be used for the specific detection of V. cholerae O139 Bengal, a PCR system was established. The specificity and sensitivity of the PCR were investigated by analyzing 240 strains within the family Vibrionaceae and 178 stains of other gram-negative bacteria. All V. cholerae O139 Bengal strains tested were positive, and none of the 384 control strains were amplified. The sensitivity of the assay was 10(2) CFU/ml.
我们通过任意PCR分离并鉴定了霍乱弧菌O139孟加拉型特异性DNA区域。该片段包含开放阅读框，编码两种可能参与荚膜多糖或脂多糖生物合成的潜在糖基转移酶。为了评估该区域用于特异性检测霍乱弧菌O139孟加拉型的可能性，建立了一个PCR系统。通过分析弧菌科内的240株菌株和其他革兰氏阴性菌的178株菌株，研究了该PCR的特异性和敏感性。所有检测的霍乱弧菌O139孟加拉型菌株均为阳性，384株对照菌株均未扩增。该检测方法的灵敏度为10(2) CFU/ml。
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