用于特异性检测霍乱弧菌O139孟加拉型的DNA探针的评估
Evaluation of DNA probes for specific detection of Vibrio cholerae O139 Bengal.
作者信息
Nair G B, Bag P K, Shimada T, Ramamurthy T, Takeda T, Yamamoto S, Kurazono H, Takeda Y
机构信息
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
出版信息
J Clin Microbiol. 1995 Aug;33(8):2186-7. doi: 10.1128/jcm.33.8.2186-2187.1995.
Abstract
Two DNA probes, 2R1 and 2R3, prepared from a region in the chromosome specific for the lipopolysaccharide O side chains of Vibrio cholerae O139 (M.K. Waldor and J.J. Mekalanos, Lancet 343:1366, 1994) were examined for their specificity and sensitivity. Both probes did not hybridize with any strain of V. cholerae belonging to serogroups other than O139 and to any of the other species examined belonging to the family Vibrionaceae. Among the 126 strains of V. cholerae O139 examined, probe 2R1 hybridized with 125 strains while probe 2R3 hybridized with all 126 strains. Both probes were found to be highly specific and sensitive and can be used for the specific identification of V. cholerae O139.
摘要
对从霍乱弧菌O139脂多糖O侧链特异的染色体区域制备的两种DNA探针2R1和2R3进行了特异性和敏感性检测。这两种探针均未与除O139之外的任何霍乱弧菌血清群菌株以及弧菌科其他检测的任何物种杂交。在所检测的126株霍乱弧菌O139菌株中，探针2R1与125株杂交，而探针2R3与所有126株杂交。发现这两种探针均具有高度特异性和敏感性，可用于霍乱弧菌O139的特异性鉴定。
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