Rosencwaig A, Pines E
J Invest Dermatol. 1977 Sep;69(3):296-8. doi: 10.1111/1523-1747.ep12507522.
Conventional spectroscopic studies on opaque membranes are difficult to pursue because of excessive light scattering and complications arising from the specimen's surface. Recently, a new spectroscopic technique, photoacoustic spectroscopy, unlike conventional optical spectroscopy, has been demonstrated to be an informative technique amenable to spectroscopic studies of solids and membrane-like samples. We have applied photoacoustic spectroscopy to the study of hydration and maturation of newborn rat stratum corneum, and have obtained clean spectra in the 220 to 450 nm region indicative of a change in thermal diffusivity with increased hydration, and biochemical changes associated with the initial maturation period.
由于存在过多的光散射以及样本表面引发的复杂情况，对不透明膜进行传统光谱研究颇具难度。近来，一种新型光谱技术——光声光谱法，与传统光学光谱法不同，已被证明是一种适用于固体和膜状样本光谱研究的信息丰富的技术。我们已将光声光谱法应用于新生大鼠角质层水合作用和成熟过程的研究，并在220至450纳米区域获得了清晰的光谱，这些光谱表明随着水合作用增强热扩散率发生了变化，以及与初始成熟阶段相关的生化变化。
