Cenacchi G, Giangaspero F, Cerasoli S, Manetto V, Martinelli G N
Istituto di Microscopia Elettronica Clinica, Università di Bologna, Italy.
Ultrastruct Pathol. 1996 Nov-Dec;20(6):537-47. doi: 10.3109/01913129609016358.
Cells with uniform, small-round nucleus and clear cytoplasm (oligodendroglial-like cell, OLC) are commonly observed in central nervous system (CNS) neoplasm of glial and neuronal lineage, such as oligodendroglioma, clear-cell ependymoma, and central neurocytoma. Immunohistochemistry does not always contribute to the characterization of OLC because of (1) loss of antigen expression; (2) lack of specific markers for oligodendrogliomas; and (3) occasional coexpression of neuronal and glial antigens. An ultrastructural analysis associated with an immunohistochemical study of 20 cases of CNS tumors largely constituted by OLCs has been performed. Neurocytomas (12 cases), medullocytomas (2 cases), cerebral neuroblastoma (1 case), and ganglioglioma (1 case) showed OLCs with ultrastructural features of neuronal differentiation (neuritic processes, dense-core granules, synaptic structures). Oligodendroglioma (3 cases) OLCs were characterized by mitochondrial-rich cytoplasm, and ependymoma (1 case) OLCs showed microrosettes and scattered cilia. The electron microscopic analysis can provide a more precise diagnosis of these OLC-containing tumors despite their uniform morphological appearance.
细胞核均匀、呈小圆形且细胞质清晰的细胞（少突胶质细胞样细胞，OLC）常见于神经胶质和神经元谱系的中枢神经系统（CNS）肿瘤中，如少突胶质细胞瘤、透明细胞室管膜瘤和中枢神经细胞瘤。免疫组织化学并不总是有助于OLC的特征描述，原因如下：（1）抗原表达缺失；（2）缺乏少突胶质细胞瘤的特异性标志物；（3）偶尔会出现神经元和神经胶质抗原的共表达。我们对20例主要由OLC构成的CNS肿瘤进行了超微结构分析，并结合免疫组织化学研究。神经细胞瘤（12例）、髓细胞瘤（2例）、脑成神经细胞瘤（1例）和节细胞胶质瘤（1例）中的OLC具有神经元分化的超微结构特征（神经突、致密核心颗粒、突触结构）。少突胶质细胞瘤（3例）的OLC以富含线粒体的细胞质为特征，室管膜瘤（1例）的OLC显示有微玫瑰花结和散在的纤毛。尽管这些含OLC的肿瘤形态外观一致，但电子显微镜分析可为其提供更精确的诊断。
