Rahman S M, Eto H, Morshed S A, Itakura H
Department of Pathology, Institute of Tropical Medicine, Nagasaki University, Japan.
Ultrastruct Pathol. 1996 Nov-Dec;20(6):585-91. doi: 10.3109/01913129609016363.
An autopsy case of measles giant cell pneumonia with intranuclear inclusion bodies is reported. This case of giant cell pneumonia was studied by light microscopy and immunohistochemistry using monoclonal and polyclonal antibody to measles and by electron microscopy (EM). Light microscopic examination showed multinucleated epithelial giant cells with intranuclear and intracytoplasmic inclusions. The giant cells contained prominent, sharply marginated, eosinophilic intranuclear inclusions typical of classic measles pneumonia. Presence of measles antigen was confirmed using both monoclonal and polyclonal antibodies by peroxidase antiperoxidase method. Monoclonal antibody stained positively for intracytoplasmic and intranuclear inclusions. Electron microscopic examination of lung tissue showed intranuclear inclusions of filamentous or worm like nucleocapsid materials in multinucleated epithelial giant cells. The results suggest that this is a case of measles giant cell pneumonia and the intranuclear inclusion bodies are measles viral particles.
报告了一例伴有核内包涵体的麻疹巨细胞肺炎尸检病例。通过光学显微镜、使用针对麻疹的单克隆和多克隆抗体的免疫组织化学以及电子显微镜（EM）对该例巨细胞肺炎进行了研究。光学显微镜检查显示多核上皮巨细胞伴有核内和胞质内包涵体。这些巨细胞含有典型的经典麻疹肺炎中显著的、边界清晰的嗜酸性核内包涵体。通过过氧化物酶抗过氧化物酶法使用单克隆和多克隆抗体均证实了麻疹抗原的存在。单克隆抗体对胞质内和核内包涵体呈阳性染色。肺组织的电子显微镜检查显示多核上皮巨细胞内有丝状或蠕虫状核衣壳物质的核内包涵体。结果表明这是一例麻疹巨细胞肺炎病例，且核内包涵体为麻疹病毒颗粒。
