Da Costa J M, Neogy A B, Vouldoukis I, Silva M L, Gentilini M, Monjour L
Laboratoire de Parasitologie Experimentale, Faculte de Medicine Pitie-Salpetriere, Paris, France.
Am J Trop Med Hyg. 1996 Nov;55(5):511-5. doi: 10.4269/ajtmh.1996.55.511.
The antigenic components of a semipurified fraction of Leishmania donovani infantum were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis using 14 serum samples from dogs with symptomatic visceral leishmaniasis and 11 serum samples from apparently healthy dogs collected in an area endemic for canine visceral leishmaniasis (CVL). It was found that these antigens were composed of many polypeptides, among which seven components recognized by symptomatic CVL sera, had molecular weights of approximately 18, 28, 30, 33, 63, 70, and 72 kilodaltons (kD); two components of 63 and 70 kD were recognized by three of 11 healthly dog sera. These findings suggest that specific antigens induce humoral immune response in dogs with asymptomatic or active visceral leishmaniasis. Infected dogs are not readily identifiable by their symptoms. The potential interest of the immunoblot test for CVL diagnostic purposes is discussed.
使用来自有症状的内脏利什曼病犬的14份血清样本以及在犬内脏利什曼病（CVL）流行地区采集的11份来自看似健康犬的血清样本，通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳和蛋白质印迹分析，确定了婴儿利什曼原虫半纯化组分的抗原成分。结果发现，这些抗原由许多多肽组成，其中有7种成分能被有症状的CVL血清识别，其分子量约为18、28、30、33、63、70和72千道尔顿（kD）；63和70 kD的两种成分能被11份健康犬血清中的3份识别。这些发现表明，特定抗原可在无症状或活动性内脏利什曼病犬中诱导体液免疫反应。受感染的犬不易通过症状识别。文中讨论了免疫印迹试验用于CVL诊断目的的潜在意义。
