Rami M, Atarhouch T, Dakkak A
Institut Agronomique et Vétérinaire Hassan II, Department of Parasitology and Parasitic Diseases, BP 6202, Rabat-Instituts 10101, Morocco.
Vet Parasitol. 2005 Nov 25;134(1-2):25-31. doi: 10.1016/j.vetpar.2005.06.012. Epub 2005 Aug 1.
In the aim of improving serodiagnosis of canine leishmaniosis, we analysed the humoral immune response of dog against Leishmania infantum parasite. The antigenic reaction of L. infantum polypeptides with sera from 31 dogs with parasitologically confirmed leishmaniosis was studied by using the immunoblot technique. Electrophoretic profile of the parasite extract showed more than 50 polypeptides, with molecular weights ranging from 12 to 170 kDa. Among these polypeptides, 37 antigen components, ranging from 14 to 91 kDa, were recognised by antibodies of L. infantum infected dogs. Three polypeptides (14, 16 and 76 kDa) reacted with all of the 31 serum samples. The other most frequently recognised antigens were those of 29.5, 32, 46, 59 and 66 kDa with a sensitivity of 87.1%, 93.6%, 96.8%, 87.1% and 80.6%, respectively. The 14 and 16 kDa bands were the most intense and remained detectable until a serum dilution of 1:6400. No reaction of these two major antigens was observed with sera collected from 50 Leishmania-free dogs, living in the leishmaniosis-free region of Rabat in Morocco, whereas the crude antigen used in IFAT or ELISA lead to three false positive results. Four antigen components of 29, 41, 55, and 70 kDa were recognised by some sera samples from negative controls. These results demonstrated the potential interest of the fractions of 14 and 16 kDa in immunodiagnosis of canine leishmaniosis.
为了改进犬利什曼病的血清学诊断，我们分析了犬针对婴儿利什曼原虫寄生虫的体液免疫反应。通过免疫印迹技术研究了婴儿利什曼原虫多肽与31只经寄生虫学确诊为利什曼病的犬的血清的抗原反应。寄生虫提取物的电泳图谱显示有50多种多肽，分子量范围为12至170 kDa。在这些多肽中，37种抗原成分，分子量在14至91 kDa之间，被婴儿利什曼原虫感染犬的抗体识别。三种多肽（14、16和76 kDa）与所有31份血清样本发生反应。其他最常被识别的抗原是29.5、32、46、59和66 kDa的抗原，敏感性分别为87.1%、93.6%、96.8%、87.1%和80.6%。14和16 kDa的条带最强烈，直到血清稀释至1:6400仍可检测到。在摩洛哥拉巴特无利什曼病地区生活的50只无利什曼原虫的犬采集的血清中未观察到这两种主要抗原的反应，而间接荧光抗体试验（IFAT）或酶联免疫吸附测定（ELISA）中使用的粗抗原则导致三个假阳性结果。一些阴性对照血清样本识别出29、41、55和70 kDa的四种抗原成分。这些结果证明了14和16 kDa组分在犬利什曼病免疫诊断中的潜在价值。
