人类细胞质肌动蛋白基因ACTB定位于7p22、ACTG1定位于17q25的荧光原位杂交定位及相关假基因的特征分析
FISH localization of human cytoplasmic actin genes ACTB to 7p22 and ACTG1 to 17q25 and characterization of related pseudogenes.
作者信息
Ueyama H, Inazawa J, Nishino H, Ohkubo I, Miwa T
机构信息
Department of Medical Biochemistry, Shiga University of Medical Science, Japan.
出版信息
Cytogenet Cell Genet. 1996;74(3):221-4. doi: 10.1159/000134420.
Human beta- and gamma-cytoplasmic actin genes (ACTB and ACTG1) were mapped to chromosomes 7p22 and 17q25, respectively, by fluorescence in situ hybridization (FISH). Four processed pseudogenes, beta-actin-related ACTBP9 and gamma-actin-related ACTGP1, ACTGP3, and ACTGP9, were isolated from human libraries. By PCR of somatic cell hybrid DNAs, ACTBP9 and two beta-actin-related pseudogenes (ACTBP7 and ACTBP8) were mapped to human chromosomes 18, 15, and 6, respectively. The gamma-actin-related pseudogenes were mapped by FISH to chromosomes 3q23 (ACTGP1), 20p13 (ACTGP3), and 6p21.1 (ACTGP9).
通过荧光原位杂交（FISH）技术，人类β-和γ-细胞质肌动蛋白基因（ACTB和ACTG1）分别被定位到7号染色体p22区带和17号染色体q25区带。从人类文库中分离出了四个加工假基因，即β-肌动蛋白相关的ACTBP9和γ-肌动蛋白相关的ACTGP1、ACTGP3和ACTGP9。通过对体细胞杂种DNA进行聚合酶链反应（PCR），ACTBP9以及另外两个β-肌动蛋白相关假基因（ACTBP7和ACTBP8）分别被定位到人类18号、15号和6号染色体上。γ-肌动蛋白相关假基因通过FISH技术被定位到3号染色体q23区带（ACTGP1）、20号染色体p13区带（ACTGP3）和6号染色体p21.1区带（ACTGP9）。
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