Alsemgeest S P, van 't Klooster G A, van Miert A S, Hulskamp-Koch C K, Gruys E
Department of Pathology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Vet Immunol Immunopathol. 1996 Sep;53(1-2):179-84. doi: 10.1016/0165-2427(96)05602-4.
To investigate the utility of primary cultures of bovine hepatocytes for compartmentalized acute phase protein studies the secretion of serum amyloid-A (SAA) and haptoglobin (Hp) was measured after stimulation by pro-inflammatory cytokines (recombinant human IL-6 (rhIL-6) and recombinant human tumour necrosis factor-alpha (rhTNF-alpha)). During the incubation period of the experiment, the SAA and Hp secretion into the culture medium increased (P < 0.05). SAA concentrations showed an additional increase following treatment with each of the cytokines (P < 0.01). Hp concentrations remained unchanged, whereas incubation with a combination of both resulted in a significant increase of the medium concentration of both SAA (P < 0.01) and Hp (P < 0.05). From these findings it is concluded that primary bovine hepatocytes can be used for in vitro studies on acute-phase protein secretion.
为了研究牛肝细胞原代培养物在急性期蛋白分区研究中的实用性，在促炎细胞因子（重组人白细胞介素-6（rhIL-6）和重组人肿瘤坏死因子-α（rhTNF-α））刺激后，测量血清淀粉样蛋白A（SAA）和触珠蛋白（Hp）的分泌。在实验的孵育期内，培养基中SAA和Hp的分泌增加（P < 0.05）。用每种细胞因子处理后，SAA浓度进一步增加（P < 0.01）。Hp浓度保持不变，而两种细胞因子联合孵育导致培养基中SAA（P < 0.01）和Hp（P < 0.05）的浓度显著增加。从这些发现可以得出结论，原代牛肝细胞可用于急性期蛋白分泌的体外研究。
