Oral E, Seli E, Bahtiyar M O, Olive D L, Arici A
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut, USA.
Obstet Gynecol. 1996 Dec;88(6):1050-6. doi: 10.1016/s0029-7844(96)00361-4.
To investigate the presence and modulation of growth-regulated alpha, a member of the chemokine family with neutrophil chemotactic activity, in the peritoneal fluid of women with or without endometriosis.
Peritoneal fluid samples were obtained at laparoscopy from 63 women with endometriosis and 19 fertile women without endometriosis. Endometrial tissue was obtained from uteri after hysterectomy for reasons other than endometrial disease or from endometrial biopsies of reproductive-age women. Cellular RNA was extracted and northern blots were hybridized with an oligonucleotide probe complementary to a specific sequence of growth-regulated alpha messenger RNA. Growth-regulated alpha in peritoneal fluid and culture supernatant was quantified using enzyme-linked immunosorbent assay. Statistical analyses were performed using Kruskal-Wallis and Mann-Whitney tests.
The median (range) concentration of growth-regulated alpha in peritoneal fluid samples from 19 normal fertile women was 27 pg/mL (0-108), from 24 women with moderate endometriosis 34 pg/mL (8-150), and from seven with severe endometriosis was 73 pg/mL (10-221) (P = .04, P = .01, respectively). In the moderate and severe endometriosis groups, the levels of growth-regulated alpha were significantly higher in the peritoneal fluid of women with untreated endometriosis (73 pg/mL [10-221]) than in women with medically treated endometriosis (25 pg/mL [8-47]). In mesothelial and endometrial stromal cells in culture, growth-regulated alpha messenger RNA and protein were detectable constitutively; however, both interleukin-1 alpha and tumor necrosis factor-alpha induced higher levels of growth-regulated alpha messenger RNA and protein in a dose- and time-dependent manner.
Growth-regulated alpha levels are elevated in the peritoneal fluid of women with moderate and severe endometriosis. This chemotactic factor, which acts via the interleukin-8 receptor, may play a role in the pathogenesis of endometriosis.
研究具有中性粒细胞趋化活性的趋化因子家族成员生长调节α在有或无子宫内膜异位症女性腹腔液中的存在情况及其调节作用。
在腹腔镜检查时从63例子宫内膜异位症女性和19例未患子宫内膜异位症的可育女性获取腹腔液样本。子宫内膜组织取自因非子宫内膜疾病行子宫切除术后的子宫，或取自育龄女性的子宫内膜活检组织。提取细胞RNA，并用与生长调节α信使核糖核酸特定序列互补的寡核苷酸探针进行Northern杂交。使用酶联免疫吸附测定法对腹腔液和培养上清液中的生长调节α进行定量。采用Kruskal-Wallis检验和Mann-Whitney检验进行统计学分析。
19例正常可育女性腹腔液样本中生长调节α的中位数（范围）浓度为27 pg/mL（0 - 108），24例中度子宫内膜异位症女性为34 pg/mL（8 - 150），7例重度子宫内膜异位症女性为73 pg/mL（10 - 221）（P分别为0.04、0.01）。在中度和重度子宫内膜异位症组中，未治疗的子宫内膜异位症女性腹腔液中生长调节α的水平（73 pg/mL [10 - 221]）显著高于接受药物治疗的子宫内膜异位症女性（25 pg/mL [8 - 47]）。在培养的间皮细胞和子宫内膜基质细胞中，可组成性检测到生长调节α信使核糖核酸和蛋白质；然而，白细胞介素-1α和肿瘤坏死因子-α均以剂量和时间依赖性方式诱导更高水平的生长调节α信使核糖核酸和蛋白质。
中度和重度子宫内膜异位症女性腹腔液中生长调节α水平升高。这种通过白细胞介素-8受体起作用的趋化因子可能在子宫内膜异位症的发病机制中起作用。
