Mathias N, Johnson S L, Winey M, Adams A E, Goetsch L, Pringle J R, Byers B, Goebl M G
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202-5122, USA.
Mol Cell Biol. 1996 Dec;16(12):6634-43. doi: 10.1128/MCB.16.12.6634.
Regulation of cell cycle progression occurs in part through the targeted degradation of both activating and inhibitory subunits of the cyclin-dependent kinases. During G1, CDC4, encoding a WD-40 repeat protein, and CDC34, encoding a ubiquitin-conjugating enzyme, are involved in the destruction of these regulators. Here we describe evidence indicating that CDC53 also is involved in this process. Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo. Cdc53p defines a large family of proteins found in yeasts, nematodes, and humans whose molecular functions are uncharacterized. These results suggest a role for this family of proteins in regulating cell cycle proliferation through protein degradation.
细胞周期进程的调控部分是通过细胞周期蛋白依赖性激酶的激活亚基和抑制亚基的靶向降解来实现的。在G1期，编码WD-40重复蛋白的CDC4和编码泛素结合酶的CDC34参与了这些调节因子的破坏。在此，我们描述了表明CDC53也参与这一过程的证据。CDC53中的突变导致的表型与cdc4和cdc34突变的表型无法区分，这些基因之间存在许多遗传相互作用，并且在体内发现编码的蛋白质在物理上相互关联。Cdc53p定义了一个在酵母、线虫和人类中发现的蛋白质大家族，其分子功能尚未明确。这些结果表明该蛋白家族在通过蛋白质降解调节细胞周期增殖中发挥作用。
