National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, China.
Sci Rep. 2019 Feb 20;9(1):2406. doi: 10.1038/s41598-019-38648-x.
As post-transcriptional regulators, microRNAs (miRNAs) play an important role in growth and reproductive processes. So far, there is limited information regarding crustacean miRNAs. To explore the potential role of miRNAs in the gonadal development of the prawn Macrobrachium rosenbergii, we constructed seven small RNA libraries from ovarian and testicular tissues at various stages using somatic tissue as the control. A total of 1,954 known and 129 novel miRNAs were retrieved. By comparing differentially expressed miRNAs (DEMs) between testes and ovaries, forty-one miRNAs were identified with sex-biased expression patterns, including 17 ovary-biased and 24 testis-biased patterns. Furthermore, the putative target genes of the sex-biased miRNAs, such as cyclin L1, mitogen-activated protein kinase 7 (MAPK 7), heat shock protein (HSP), and zinc finger protein, were significantly enriched in many reproduction-related pathways including the Gonadotropin-releasing hormone (GnRH) pathway, glycolysis, gluconeogenesis pathway, ovarian steroidogenesis, estrogen signaling pathway, MAPK pathway, Wnt pathway, and insulin signaling pathway, implicating potential regulatory roles of miRNAs in reproduction. These data aid in the further investigation of the mechanism of gonadal development and reproductive regulation mediated by miRNA in M. rosenbergii.
作为转录后调控因子，microRNAs（miRNAs）在生长和生殖过程中发挥着重要作用。迄今为止，有关甲壳动物 miRNAs 的信息有限。为了探讨 miRNAs 在对虾Macrobrachium rosenbergii 性腺发育中的潜在作用，我们使用体组织作为对照，从卵巢和睾丸组织的不同阶段构建了七个小 RNA 文库。共检索到 1954 个已知和 129 个新的 miRNAs。通过比较睾丸和卵巢之间差异表达的 miRNAs（DEMs），鉴定出 41 个具有性别偏表达模式的 miRNAs，包括 17 个卵巢偏表达和 24 个睾丸偏表达模式。此外，这些性别偏表达 miRNAs 的潜在靶基因，如细胞周期蛋白 L1、丝裂原激活蛋白激酶 7（MAPK 7）、热休克蛋白（HSP）和锌指蛋白，在许多与生殖相关的途径中显著富集，包括促性腺激素释放激素（GnRH）途径、糖酵解、糖异生途径、卵巢类固醇生成、雌激素信号通路、MAPK 通路、Wnt 通路和胰岛素信号通路，表明 miRNA 在生殖过程中可能具有调节作用。这些数据有助于进一步研究 miRNA 在 M. rosenbergii 性腺发育和生殖调控中的作用机制。
