人精浆中苗勒管抑制物质的存在。

The presence of müllerian inhibiting substance in human seminal plasma.

作者信息

Fallat M E, Siow Y, Belker A M, Boyd J K, Yoffe S, MacLaughlin D T

机构信息

Department of Surgery, University of Louisville, KY, USA.

出版信息

Hum Reprod. 1996 Oct;11(10):2165-9. doi: 10.1093/oxfordjournals.humrep.a019070.

DOI:10.1093/oxfordjournals.humrep.a019070

PMID:8943523

Abstract

Müllerian inhibiting substance (MIS), produced by testicular Sertoli cells, is present in adult male serum. The first aim of this study was to determine if MIS is present in seminal plasma. Using an enzyme-linked immunosorbent assay (ELISA), we measured MIS concentrations in seminal plasma from 23 donors exhibiting normal (WHO criteria) sperm qualities, and 169 patients with subnormal sperm parameters. The second aim of this study was to examine a potential relationship between MIS and sperm motility. MIS concentrations in seminal plasma ranged from 0.5 to 3.6 ng/ml in donors and from 0.5 to 17.8 ng/ml in patients. Motility index (MI, mean +/- SEM) for all patient samples was lower compared with donors (113.3 +/- 3.2 and 198.3 +/- 13.5, P < 0.00001), while mean MIS concentration +/- SEM) was higher (4.2 +/- 0.3 and 1.4 +/- 0.2, P < 0.0003). When the patients were stratified into Groups I (motility < 50%, n = 42) and II (motility > 50%, n = 127), the MI (mean +/- SEM) values were 62.3 +/- 3.8 and 130.2 +/- 2.7 respectively (P < 0.0001 for both compared with donors) and mean MIS concentrations (+/- SEM) were 5.4 +/- 0.6 and 3.9 +/- 0.3, respectively (P < 0.0001 and P < 0.001 compared with donors). The inverse relationship between MIS concentration in seminal plasma and motility index suggests that MIS may have a function in modulating motility.

摘要

苗勒管抑制物质（MIS）由睾丸支持细胞产生，存在于成年男性血清中。本研究的首要目的是确定MIS是否存在于精浆中。我们采用酶联免疫吸附测定（ELISA）法，检测了23名精子质量正常（符合世界卫生组织标准）的供者以及169名精子参数低于正常水平的患者精浆中的MIS浓度。本研究的第二个目的是探究MIS与精子活力之间的潜在关系。供者精浆中MIS浓度范围为0.5至3.6 ng/ml，患者精浆中MIS浓度范围为0.5至17.8 ng/ml。所有患者样本的活力指数（MI，平均值±标准误）低于供者（分别为113.3±3.2和198.3±13.5，P<0.00001），而平均MIS浓度（±标准误）则高于供者（分别为4.2±0.3和1.4±0.2，P<0.0003）。当将患者分为I组（活力<50%，n = 42）和II组（活力>50%，n = 127）时，MI（平均值±标准误）值分别为62.3±3.8和130.2±2.7（与供者相比，两组P均<0.0001），平均MIS浓度（±标准误）分别为5.4±0.6和3.9±0.3（与供者相比，P分别<0.0001和P<0.001）。精浆中MIS浓度与活力指数之间的负相关关系表明，MIS可能在调节精子活力方面发挥作用。