Damiano E R, Westheider J, Tözeren A, Ley K
Department of Biomedical Engineering, University of Virginia, Charlottesville 22908, USA.
Circ Res. 1996 Dec;79(6):1122-30. doi: 10.1161/01.res.79.6.1122.
Leukocyte rolling along the endothelium in inflammation is caused by continuous formation and breakage of bonds between selectin adhesion molecules and their ligands. We investigated trauma-induced leukocyte rolling in venules (diameter, 23 to 58 microns; wall shear stress, 1.2 to 35 dyne/cm2) of the exteriorized rat mesentery using high-resolution intravital microscopy. While rolling, the leukocytes deformed into a tear-droplike shape. Deformation continued to increase with shear stress up to the highest values observed (35 dyne/cm2). Successive leukocytes had similar rolling velocities at the same axial positions along each vessel, suggesting that heterogeneity of endothelial adhesiveness is responsible for velocity variation. Adhesion energy density varied inversely with instantaneous rolling velocity and directly with instantaneous deformation. Adhesion energy density reached a maximum of 0.36 dyne/cm, similar to values found for lymphocyte function-associated antigen-1-dependent adhesion of stimulated T cells to isolated intercellular adhesion molecule-1. We conclude that selectin-mediated adhesion during rolling produces adhesion energy densities comparable to those observed for integrin-mediated adhesion events in other experimental systems.
炎症过程中白细胞在内皮上滚动是由选择素粘附分子与其配体之间不断形成和断裂的键所引起的。我们使用高分辨率活体显微镜研究了大鼠肠系膜外翻小静脉（直径23至58微米；壁面剪应力1.2至35达因/平方厘米）中创伤诱导的白细胞滚动。滚动时，白细胞变形为泪滴状。随着剪应力增加至观察到的最高值（35达因/平方厘米），变形持续增大。沿着每条血管的相同轴向位置，相继的白细胞具有相似的滚动速度，这表明内皮粘附性的异质性是速度变化的原因。粘附能量密度与瞬时滚动速度成反比，与瞬时变形成正比。粘附能量密度最高达到0.36达因/厘米，类似于刺激的T细胞与分离的细胞间粘附分子-1的淋巴细胞功能相关抗原-1依赖性粘附所发现的值。我们得出结论，滚动过程中选择素介导的粘附产生的粘附能量密度与其他实验系统中整合素介导的粘附事件所观察到的相当。
