Iwahashi M, Ooshima A, Nakano R
Department of Obstetrics and Gynaecology, Wakayama Medical College, Japan.
J Clin Pathol. 1996 Nov;49(11):916-9. doi: 10.1136/jcp.49.11.916.
To obtain some insight into the extracellular matrix in the placenta, changes in the composition of collagens during placental development were investigated.
Collagen was extracted from placentas (group 1, 25-30 weeks, n = 21; group 2, 31-36 weeks, n = 32; and group 3, 37-41 weeks of gestation, n = 40) and the relative concentrations of various collagens were evaluated by SDS-PAGE.
The ratio of the intensity of the alpha 1 (III) band to that of alpha 1 (I) chain collagen in group 3 placentas were lower than those in group 1 placentas. In contrast, the ratio of the intensity of the alpha 1 (V) band to that of alpha 1 (I) chain collagen in group 3 placentas were higher than those in group 1 and group 2 placentas.
These results suggest that type V collagen might play an important role in the function of the placenta and that an increased relative concentration of type V collagen might be closely associated with the development and ageing of the placenta.
为深入了解胎盘的细胞外基质，研究胎盘发育过程中胶原蛋白组成的变化。
从胎盘（第1组，孕25 - 30周，n = 21；第2组，孕31 - 36周，n = 32；第3组，孕37 - 41周，n = 40）中提取胶原蛋白，并通过SDS - PAGE评估各种胶原蛋白的相对浓度。
第3组胎盘α1（III）带强度与α1（I）链胶原蛋白强度之比低于第1组胎盘。相反，第3组胎盘α1（V）带强度与α1（I）链胶原蛋白强度之比高于第1组和第2组胎盘。
这些结果表明V型胶原蛋白可能在胎盘功能中起重要作用，且V型胶原蛋白相对浓度的增加可能与胎盘的发育和老化密切相关。
