Zaidan E, Sims N R
Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, Australia.
Neurosci Lett. 1996 Nov 1;218(2):75-78. doi: 10.1016/s0304-3940(96)13128-1.
Total glutathione was measured in mitochondria isolated following 30 min of ischemia and recirculation periods up to 24 h. Mitochondria prepared from the dorsolateral striatum, a region containing many neurons susceptible to short ischemic periods, were compared with those from the paramedian cortex, an ischemia-resistant region. Parallel increases in glutathione content (to approximately 150% of pre-ischemic values) were seen in both regions during the first few hours of recirculation. By 24 h of recirculation, there was a decrease below pre-ischemic values in preparations from the dorsolateral striatum but not the paramedian cortex. The early increases in mitochondrial glutathione were not associated with comparable increases in total tissue glutathione. A shorter (10 min) ischemic period also produced an early increase in mitochondrial glutathione but this was reversed more rapidly to preischemic values. The observed changes indicate post-ischemic modifications of cellular oxidative defenses in the two brain regions studied.
在缺血30分钟后分离出线粒体，并在长达24小时的再灌注期内测定总谷胱甘肽。将从背外侧纹状体（该区域含有许多易受短暂缺血影响的神经元）制备的线粒体与从中线旁皮质（一个抗缺血区域）制备的线粒体进行比较。在再灌注的最初几个小时内，两个区域的谷胱甘肽含量均平行增加（达到缺血前值的约150%）。到再灌注24小时时，背外侧纹状体的制剂中谷胱甘肽含量降至缺血前值以下，而中线旁皮质则没有。线粒体谷胱甘肽的早期增加与总组织谷胱甘肽的相应增加无关。较短的（10分钟）缺血期也会使线粒体谷胱甘肽早期增加，但这种增加会更快地恢复到缺血前值。观察到的变化表明，在所研究的两个脑区中，细胞氧化防御发生了缺血后修饰。
