大鼠虹膜和睫状体上皮中巨噬细胞移动抑制因子的检测与免疫定位

Detection and immunolocalization of macrophage migration inhibitory factor in rat iris and ciliary epithelium.

作者信息

Matsuda A, Kotake S, Tagawa Y, Matsuda H, Nishihira J

机构信息

Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.

出版信息

Immunol Lett. 1996 Oct;53(1):1-5. doi: 10.1016/0165-2478(96)02600-4.

DOI:10.1016/0165-2478(96)02600-4

PMID:8946210

Abstract

To elucidate the role of macrophage migration inhibitory factor (MIF) in ocular inflammation, we examined the localization of MIF in the normal anterior uveal tract of rats. Immunohistochemistry using an anti-MIF antibody revealed that MIF was present in non-pigment epithelial cells of the ciliary body and the epithelial cells of the iris. Western blot analysis of these tissues showed a single band specific for MIF protein. The expression of MIF mRNA in these tissues was further confirmed by reverse transcription-polymerase chain reaction. Since MIF is known to be a potent proinflammatory cytokine, identification of the protein in iris and ciliary epithelial cells suggests the possibility that it may play an important role in ocular inflammation.

摘要

为阐明巨噬细胞移动抑制因子（MIF）在眼部炎症中的作用，我们检测了MIF在正常大鼠前葡萄膜中的定位。使用抗MIF抗体进行免疫组织化学分析显示，MIF存在于睫状体的非色素上皮细胞和虹膜的上皮细胞中。对这些组织进行的蛋白质印迹分析显示出一条对MIF蛋白特异的条带。通过逆转录-聚合酶链反应进一步证实了这些组织中MIF mRNA的表达。由于已知MIF是一种强效促炎细胞因子，在虹膜和睫状体上皮细胞中鉴定出该蛋白提示其可能在眼部炎症中发挥重要作用。