Naruse K, Nagashima I, Sakai Y, Harihara Y, Jiang G X, Suzuki M, Muto T, Makuuchi M
Department of Surgery, Faculty of Medicine, University of Tokyo, Japan.
Artif Organs. 1998 Dec;22(12):1031-7. doi: 10.1046/j.1525-1594.1998.06104.x.
We developed a new bioreactor for a bioartificial liver filled with porcine hepatocytes immobilized on polyester nonwoven fabric (NWF) and in our previous study showed that this NWF bioreactor has promising in vitro efficiency. In the present study, we investigated the efficacy of the NWF bioreactor in a direct hemoperfusion experiment conducted to treat pigs with liver failure. Porcine hepatocytes were isolated from the whole liver of a Sangen strain pig. They were immobilized in a 200 ml column containing NWF via perfusion in a closed circuit for 24 h to prepare a NWF bioreactor. The following day an operative liver failure model was produced by creating a portocaval shunt and ligating the entire hepatoduodenal ligament in the porta hepatis. Perfusion treatment was initiated 4 h after operative induction of liver failure and continued for about 1 h. The pigs which underwent perfusion treatment showed significant improvements in survival and blood data, including ammonia, total bile acid, glucose, and prothrombin time, attributed to significant improvements in the post- as compared to the prebioreactor levels in the perfused blood of the treated pigs. These beneficial effects of the NWF bioreactor were based on its excellent composition which allows the accommodation of adequate numbers of hepatocytes and direct contact between hepatocytes and perfused blood.
我们开发了一种用于生物人工肝的新型生物反应器，其填充有固定在聚酯无纺布（NWF）上的猪肝细胞，并且在我们之前的研究中表明这种NWF生物反应器具有良好的体外效率。在本研究中，我们在一项用于治疗肝功能衰竭猪的直接血液灌注实验中研究了NWF生物反应器的功效。从三穗品系猪的全肝中分离猪肝细胞。通过在封闭回路中灌注24小时，将它们固定在一个装有NWF的200毫升柱中，以制备NWF生物反应器。第二天，通过建立门腔分流并结扎肝门处的整个肝十二指肠韧带，建立手术性肝功能衰竭模型。在手术诱导肝功能衰竭4小时后开始灌注治疗，并持续约1小时。接受灌注治疗的猪在存活率和血液数据方面有显著改善，包括氨、总胆汁酸、葡萄糖和凝血酶原时间，这归因于与治疗猪灌注血液中生物反应器前水平相比，生物反应器后水平有显著改善。NWF生物反应器的这些有益效果基于其优良的组成，该组成允许容纳足够数量的肝细胞，并使肝细胞与灌注血液直接接触。
