Honkanen R E, Mowdy D E, Dickey R W
University of South Alabama, College of Medicine, Department of Biochemistry and Molecular Biology, Mobile 36688, USA.
J AOAC Int. 1996 Nov-Dec;79(6):1336-43.
A relatively rapid protein phosphatase-based assay was developed for detecting okadaic acid in extracts of shellfish, using oysters (Crassostrea virginica) as a model. The assay has good sensitivity, detecting okadaic acid in crude methanolic extracts of oysters at > or = 4 ng/mL (> or = 0.1 ng/assay). Assay accuracy for detecting toxic shellfish was validated through a series of spike recovery experiments. In more than 320 assessments, all oysters containing toxic amounts of okadaic acid (> or = 0.2 microgram/g) were detected. Results of analysis of the same extract by phosphatase assay and liquid chromatography gave very high correlation.
开发了一种基于蛋白磷酸酶的相对快速的检测方法，以牡蛎（弗吉尼亚巨蛎）为模型检测贝类提取物中的冈田酸。该检测方法具有良好的灵敏度，能检测出牡蛎粗甲醇提取物中浓度≥4 ng/mL（≥0.1 ng/检测）的冈田酸。通过一系列加标回收实验验证了检测有毒贝类的方法准确性。在320多次评估中，所有含有有毒量冈田酸（≥0.2微克/克）的牡蛎均被检测出来。磷酸酶检测法和液相色谱法对同一提取物的分析结果具有很高的相关性。
