Waisel Y, Geller-Bernstein C, Keynan N, Arad G
Department of Botany, Tel-Aviv University, Israel.
Allergy. 1996 Nov;51(11):819-25. doi: 10.1111/j.1398-9995.1996.tb00028.x.
The incidence of positive skin test responses among atopic subjects with suspected respiratory allergy was investigated with commercial and autochthonous pollen extracts of various cultivars of Olea europaea. Pollen was collected from olive trees of well-defined cultivars, extracted, and separated by SDS-PAGE. Immunoblots were used to identify the various IgE-binding proteins of the pollen extracts of the various cultivars. The results revealed six predominant IgE-binding bands, some of which appear in all the cultivars examined. The 18-20-kDa band (Ole e 1) appeared in only eight of the cultivars, but not in the nine others. The presence of specific IgE-binding bands in the various pollen extracts and their correlation with the incidence of positive skin tests are discussed.
采用市售和本地不同品种油橄榄的花粉提取物，对疑似呼吸道过敏的特应性受试者进行皮肤试验阳性反应发生率的调查。花粉从明确品种的橄榄树上采集，经提取后通过SDS-PAGE进行分离。免疫印迹法用于鉴定不同品种花粉提取物中各种IgE结合蛋白。结果显示有六条主要的IgE结合带，其中一些出现在所有检测的品种中。18 - 20 kDa的条带（油橄榄属第1类过敏原）仅在八个品种中出现，而在其他九个品种中未出现。讨论了不同花粉提取物中特异性IgE结合带的存在情况及其与皮肤试验阳性发生率的相关性。
