Pajarón M J, Vila L, Prieto I, Resano A, Sanz M L, Oehling A K
Department of Allergology and Clinical Immunology, Faculty of Medicine, University of Navarra, Pamplona, Spain.
Allergy. 1997 Aug;52(8):829-35. doi: 10.1111/j.1398-9995.1997.tb02154.x.
Cross-reactivity between pollen extracts of four species of Oleaceae was studied: olive (Olea europaea), ash (Fraxinus excelsior), privet (Ligustrum vulgare), and lilac (Syringa vulgaris). Thus, 51 patients and 13 atopic controls were studied, by means of intracutaneous skin tests, histamine-release tests against the four extracts, and specific IgE to O. europaea. The proteic content of the four extracts was assessed by SDS-PAGE and immunoblotting, and similarity of all the extracts studied was observed after electrophoresis and immunodetection. Six common bands were found to be responsible for the cross-reactivity, with apparent molecular weights of 49.6, 40, 36.7, 19.7, 16.7, and 14 kDa, respectively. The cross-reactivity was also corroborated by immunoblotting inhibition and FEIA inhibition. The patients had a similar response to the four allergenic extracts used, although the response to Olea was greatest. When the patients were compared by their geographic origin (northern or southern Spain, according to the distribution of areas of olive pollen influence), there were no significant differences between the two groups in skin reactivity, but a higher histamine release was observed for the four extracts in the southern group, although it was significant only for Fraxinus and Ligustrum. This work corroborated the practicality of the diagnostic methods used and the cross-reactivity between the four species studied, as demonstrated by the different methods used. Therefore, we suggest that only O. europaea extract be used in diagnosis and immunotherapy in Oleaceae pollen allergy.
橄榄（油橄榄）、白蜡树（欧洲白蜡树）、女贞（欧洲女贞）和丁香（紫丁香）。因此，通过皮内皮肤试验、针对这四种提取物的组胺释放试验以及对油橄榄的特异性IgE，对51名患者和13名特应性对照者进行了研究。通过SDS-PAGE和免疫印迹法评估了这四种提取物的蛋白质含量，并且在电泳和免疫检测后观察到所有研究提取物之间的相似性。发现有六条共同条带导致交叉反应性，其表观分子量分别为49.6、40、36.7、19.7、16.7和14 kDa。免疫印迹抑制和FEIA抑制也证实了交叉反应性。患者对所使用的四种变应原提取物有相似的反应，尽管对油橄榄的反应最大。当根据橄榄花粉影响区域的分布将患者按地理来源（西班牙北部或南部）进行比较时，两组在皮肤反应性方面没有显著差异，但在南部组中观察到四种提取物的组胺释放更高，尽管仅对白蜡树和女贞具有显著性。这项工作证实了所使用诊断方法的实用性以及所研究四种植物之间的交叉反应性，这已通过所使用的不同方法得到证明。因此，我们建议在木犀科花粉过敏的诊断和免疫治疗中仅使用油橄榄提取物。
