Kruse C, Grünweller A, Notbohm H, Kügler S, Purschke W G, Müller P K
Department of Medical Molecular Biology, Medical University of Lübeck, Germany.
Biochem J. 1996 Nov 15;320 ( Pt 1)(Pt 1):247-52. doi: 10.1042/bj3200247.
Vigilin, a protein found predominantly in cells and tissues with a high biosynthetic capacity, was isolated in its native form from human HEp-2 cells (A.T.C.C. CCL23) by immunoaffinity chromatography. Vigilin forms part of a novel ribonucleoprotein complex that also contains additional, as yet uncharacterized, proteins. Experimental evidence suggests that the nucleic acids entrapped in this complex are protected from RNase and belong to the tRNA family. Using either a pool of total human RNA or radioactively labelled tRNA (tRNA (Asp**)) in rebinding experiments, we could show that tRNA is selectively recaptured by the RNA-depleted vigilin-containing complex.
vigilin是一种主要存在于具有高生物合成能力的细胞和组织中的蛋白质，通过免疫亲和色谱法从人HEp-2细胞（美国典型培养物保藏中心CCL23）中以天然形式分离出来。vigilin是一种新型核糖核蛋白复合物的一部分，该复合物还包含其他尚未鉴定的蛋白质。实验证据表明，包裹在该复合物中的核酸受到RNase的保护，属于tRNA家族。在重结合实验中，使用总人类RNA池或放射性标记的tRNA（tRNA（Asp**）），我们可以证明tRNA被不含RNA的含vigilin复合物选择性地重新捕获。
