一种包含KH多结构域蛋白vigilin的新型细胞质tRNA-蛋白质复合物的证据。
Evidence for a novel cytoplasmic tRNA-protein complex containing the KH-multidomain protein vigilin.
作者信息
Kruse C, Grünweller A, Notbohm H, Kügler S, Purschke W G, Müller P K
机构信息
Department of Medical Molecular Biology, Medical University of Lübeck, Germany.
出版信息
Biochem J. 1996 Nov 15;320 ( Pt 1)(Pt 1):247-52. doi: 10.1042/bj3200247.
Abstract
Vigilin, a protein found predominantly in cells and tissues with a high biosynthetic capacity, was isolated in its native form from human HEp-2 cells (A.T.C.C. CCL23) by immunoaffinity chromatography. Vigilin forms part of a novel ribonucleoprotein complex that also contains additional, as yet uncharacterized, proteins. Experimental evidence suggests that the nucleic acids entrapped in this complex are protected from RNase and belong to the tRNA family. Using either a pool of total human RNA or radioactively labelled tRNA (tRNA (Asp**)) in rebinding experiments, we could show that tRNA is selectively recaptured by the RNA-depleted vigilin-containing complex.
摘要
vigilin是一种主要存在于具有高生物合成能力的细胞和组织中的蛋白质，通过免疫亲和色谱法从人HEp-2细胞（美国典型培养物保藏中心CCL23）中以天然形式分离出来。vigilin是一种新型核糖核蛋白复合物的一部分，该复合物还包含其他尚未鉴定的蛋白质。实验证据表明，包裹在该复合物中的核酸受到RNase的保护，属于tRNA家族。在重结合实验中，使用总人类RNA池或放射性标记的tRNA（tRNA（Asp**）），我们可以证明tRNA被不含RNA的含vigilin复合物选择性地重新捕获。
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2
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本文引用的文献
1
Vigilin is a cytoplasmic protein. A study on its expression in primary cells and in established cell lines of different species.维吉林是一种细胞质蛋白。一项关于其在不同物种原代细胞和已建立细胞系中表达的研究。
Eur J Biochem. 1993 Apr 15;213(2):727-36. doi: 10.1111/j.1432-1033.1993.tb17813.x.
2
The KH domain occurs in a diverse set of RNA-binding proteins that include the antiterminator NusA and is probably involved in binding to nucleic acid.KH结构域存在于多种RNA结合蛋白中，包括抗终止因子NusA，可能参与与核酸的结合。
FEBS Lett. 1993 Jun 21;324(3):361-6. doi: 10.1016/0014-5793(93)80152-k.
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Expression of vigilin in chicken cartilage and bone.维吉宁蛋白在鸡软骨和骨骼中的表达。
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4
Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome.KH结构域在RNA结合中的重要作用：FMR1基因的KH结构域发生突变导致脆性X综合征，该突变会损害RNA结合。
Cell. 1994 Apr 8;77(1):33-9. doi: 10.1016/0092-8674(94)90232-1.
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Conserved structures and diversity of functions of RNA-binding proteins.RNA结合蛋白的保守结构与功能多样性
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FMR1 protein: conserved RNP family domains and selective RNA binding.脆性X智力低下蛋白1：保守的核糖核蛋白家族结构域与选择性RNA结合。
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