Plenz G, Gan Y, Raabe H M, Müller P K
Institut für Medizinische Molekularbiologie, Lübeck, Germany.
Cell Tissue Res. 1993 Aug;273(2):381-9. doi: 10.1007/BF00312841.
The expression of vigilin was followed during chick embryonal development by in situ hybridization. Vigilin mRNA is abundantly expressed in tissues of mesenchymal and ectomesenchymal origin. The mesenchymal primordial cells of cartilage and bone did not show any significant expression of vigilin. As tissue differentiation proceeded, vigilin mRNA levels increased in hyaline cartilage and in both endochondral as well as intramembranous bone. The results suggest that the expression of vigilin mRNA in cartilage- and bone-forming cells, chondrocytes and osteoblasts, is dependent on the stage of development and cellular differentiation, although not a unique process of bone formation. Most striking is the correlation of the maximum vigilin mRNA expression in osteoblasts and hypertrophic chondrocytes to periods when cell-specific genes were highly transcribed and substantially translated, e.g., synthesis of procollagen and formation of extracellular matrix in bone and cartilage.
通过原位杂交技术追踪了鸡胚胎发育过程中vigilin的表达情况。Vigilin mRNA在间充质和外间充质来源的组织中大量表达。软骨和骨的间充质原始细胞未显示vigilin的任何显著表达。随着组织分化的进行，透明软骨以及软骨内成骨和膜内成骨中的vigilin mRNA水平均升高。结果表明，vigilin mRNA在软骨形成细胞、软骨细胞和成骨细胞中的表达取决于发育阶段和细胞分化，尽管它并非骨形成的独特过程。最引人注目的是，成骨细胞和肥大软骨细胞中vigilin mRNA的最大表达与细胞特异性基因高度转录和大量翻译的时期相关，例如骨和软骨中前胶原的合成以及细胞外基质的形成。
