Höidén I, Möller G
Department of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
Scand J Immunol. 1996 Nov;44(5):501-5. doi: 10.1046/j.1365-3083.1996.d01-339.x.
Purified CD8+ T cells were recently shown to produce TH1 as well as TH2 types of cytokines upon restimulation, indicating an important role for these cells in regulation of immune responses. However, it is not known if the CD8+ cells would contribute to cytokine production in the presence of cytokine secreting CD4+ cells. In the present study the authors have investigated the proportion of cytokine-producing CD4+ and CD8+ cells in the spleen after in vitro or in vivo stimulation. They found that stimulation of spleen cells with the superantigen Staphylococcal Enterotoxin B (SEB) in the presence of IL4 promoted production of IL10 and IFN gamma predominately by CD8+ cells. In contrast, the production of IL4 was almost exclusively confined to the CD4+ subset. When priming with SEB in vivo before subsequent restimulation in vitro, a protocol previously shown to induce anergy, up to 80% of the IL10 and IFN gamma positive cell expressed the CD8 marker. Taken together, these results emphasize the important role of cytokine-producing CD8+ cells and indicate that CD4+ and CD8+ T cells may, in a given situation, produce distinct cytokines.
最近研究表明，纯化的CD8 + T细胞在再次刺激后可产生TH1和TH2型细胞因子，表明这些细胞在免疫反应调节中起重要作用。然而，尚不清楚在存在分泌细胞因子的CD4 +细胞的情况下，CD8 +细胞是否会促进细胞因子的产生。在本研究中，作者研究了体外或体内刺激后脾脏中产生细胞因子的CD4 +和CD8 +细胞的比例。他们发现，在IL4存在下用超抗原葡萄球菌肠毒素B（SEB）刺激脾细胞，主要促进CD8 +细胞产生IL10和IFNγ。相反，IL4的产生几乎完全局限于CD4 +亚群。当在体外随后再次刺激之前在体内用SEB进行致敏时（先前已证明该方案可诱导无反应性），高达80％的IL10和IFNγ阳性细胞表达CD8标记。综上所述，这些结果强调了产生细胞因子的CD8 +细胞的重要作用，并表明在特定情况下，CD4 +和CD8 + T细胞可能产生不同的细胞因子。
