Moriwaki A, Wang J B, Svingos A, van Bockstaele E, Cheng P, Pickel V, Uhl G R
Molecular Neurobiology Branch, National Institute on Drug Abuse, NIH, Baltimore, Maryland, USA.
Neurochem Res. 1996 Nov;21(11):1315-31. doi: 10.1007/BF02532373.
Immunoreactivity corresponding to the C-terminus of the rat mu opiate receptor can be detected by light microscopy in fiber- and terminal-like patterns in a number of rat brain and spinal cord regions, and in immunoreactive perikarya in several of these regions. Especially abundant fiber- and terminal-like patterns were localized to superficial layers of the spinal cord dorsal horn and nucleus caudalis of the spinal tract of the trigeminal, the nucleus of the solitary tract, nucleus ambiguous, locus coeruleus, interpeduncular nucleus, medial aspect of the lateral habenular nucleus, presumed "striasomes" of the caudate-putamen and nucleus accumbens. Moderate fiber and terminal densities were found in the ventral tegmental area, more medial aspects of the thalamus and hypothalamus, and several amygdaloid nuclei. Immunostained perikarya were prominent in the nucleus accumbens and also observed in the middle layers of the cerebral cortex, septum and diagonal band, preoptic area, medial thalamic and habenular nuclei, locus coeruleus, nucleus ambiguous, nucleus of the solitary tract, trigeminal nucleus caudalis, and spinal cord substantia gelatinosa zones. Many of these localizations correspond well with the previously-determined autoradiographic distributions of mu opiate receptor ligand binding, and with reports of mu opiate receptor immunoreactivity determined using other antisera. Electron microscopic immunohistochemical studies reveal details of the membrane distribution of the mu receptor in nucleus accumbens, caudate/putamen, locus coeruleus, and spinal cord. These results suggest largely neuronal and largely extrasynaptic distributions of mu receptors that show differential patterns of perikaryal, dendritic, and/or axonal immunostaining in different central nervous system zones. Identification of these distributions adds substantially to data identifying the cellular localization of the principal opiate receptor involved in both analgesic and addictive processes.
在大鼠脑和脊髓的多个区域，通过光学显微镜可检测到与大鼠μ阿片受体C端相对应的免疫反应性，其呈现为纤维状和终末样模式，并且在其中一些区域的免疫反应性核周体中也可检测到。特别丰富的纤维状和终末样模式定位于脊髓背角浅层、三叉神经脊髓束尾侧核、孤束核、疑核、蓝斑、脚间核、外侧缰核内侧部、尾状核 - 壳核和伏隔核的假定“纹状体”。在腹侧被盖区、丘脑和下丘脑更内侧区域以及几个杏仁核中发现中等密度的纤维和终末。免疫染色的核周体在伏隔核中很突出，在大脑皮质中层、隔区和斜角带、视前区、丘脑内侧核和缰核、蓝斑、疑核、孤束核、三叉神经尾侧核以及脊髓胶状质区也可观察到。这些定位中的许多与先前确定的μ阿片受体配体结合的放射自显影分布以及使用其他抗血清确定的μ阿片受体免疫反应性报告非常吻合。电子显微镜免疫组织化学研究揭示了μ受体在伏隔核、尾状核/壳核、蓝斑和脊髓中的膜分布细节。这些结果表明μ受体主要分布在神经元中且大多位于突触外，在不同的中枢神经系统区域呈现出核周体、树突和/或轴突免疫染色的不同模式。这些分布的鉴定极大地丰富了确定参与镇痛和成瘾过程的主要阿片受体细胞定位的数据。
