Buffer may be the critical factor in measurement of anti-prothrombin antibody on a gamma-ray-irradiated plate by enzyme-linked immunosorbent assay.

作者信息

Matsuda J, Saitoh N, Tsukamoto M, Gotoh M, Gohchi K, Kawasugi K

机构信息

Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.

出版信息

Am J Hematol. 1996 Dec;53(4):242-4. doi: 10.1002/(SICI)1096-8652(199612)53:4<242::AID-AJH6>3.0.CO;2-Y.

We investigated the influence of different buffers (Tris-buffer and phosphate buffered saline (PBS)/Tween-20 buffer) on anti-prothrombin antibody (aPT) measurement by enzyme-linked immunosorbent assay (ELISA), employing a gamma-ray-irradiated plate. We found considerable discrepancies in aPT positivity between each buffer, and we suggest that the use of Tris-buffer is not suitable for aPT measurement with a gamma-ray-irradiated plate to measure aPT.