在通过酶联免疫吸附测定法在γ射线辐照板上测量抗凝血酶原抗体时，缓冲液可能是关键因素。
Buffer may be the critical factor in measurement of anti-prothrombin antibody on a gamma-ray-irradiated plate by enzyme-linked immunosorbent assay.
作者信息
Matsuda J, Saitoh N, Tsukamoto M, Gotoh M, Gohchi K, Kawasugi K
机构信息
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
出版信息
Am J Hematol. 1996 Dec;53(4):242-4. doi: 10.1002/(SICI)1096-8652(199612)53:4<242::AID-AJH6>3.0.CO;2-Y.
We investigated the influence of different buffers (Tris-buffer and phosphate buffered saline (PBS)/Tween-20 buffer) on anti-prothrombin antibody (aPT) measurement by enzyme-linked immunosorbent assay (ELISA), employing a gamma-ray-irradiated plate. We found considerable discrepancies in aPT positivity between each buffer, and we suggest that the use of Tris-buffer is not suitable for aPT measurement with a gamma-ray-irradiated plate to measure aPT.
我们使用经伽马射线辐照的酶标板，通过酶联免疫吸附测定（ELISA）研究了不同缓冲液（Tris缓冲液和磷酸盐缓冲盐水（PBS）/吐温20缓冲液）对抗凝血酶原抗体（aPT）测量的影响。我们发现每种缓冲液之间aPT阳性存在显著差异，并且我们认为使用Tris缓冲液不适合用经伽马射线辐照的酶标板测量aPT。
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