Hoshi S, Orikasa S, Takahashi T, Suzuki K, Kaneda T, Saitoh H, Yoshikawa K, Ono K, Hikichi Y, Ohkawa A, Funato T
Department of Urology, Tohoku University School of Medicine.
Hinyokika Kiyo. 1996 Oct;42(10):781-5.
We have developed a highly sensitive method to detect pelvic lymph node metastasis using the reverse transcriptase-polymerase chain reaction (RT-PCR) with primers specific for prostate-specific antigen (PSA) gene. Fine needle aspiration biopsy (FNAB) of pelvic lymph nodes was performed in 24 patients with prostate cancer. Each aspirated sample (0.05-0.1 ml) was divided into 2 parts; one for RNA extraction and RT-PCR to detect the fragment of PSA mRNA, and the other to smear on a slide glass for conventional cytology. The PSA gene was detected by RT-PCR in 11 FNAB samples which included not only all 6 cytologically positive and 2 cytologically class III cases but also 3 of 16 cytologically negative cases. The PSA gene was not detected by RT-PCR of FNAB samples in any of the 20 cases of bladder cancer. Thus RT-PCR for detection of the PSA gene in FNAB samples may be useful as a new diagnostic technique for detection of early lymph node metastasis in prostate cancer and an additional tool for cytological diagnosis of prostate cancer.
我们已经开发出一种高灵敏度的方法，使用针对前列腺特异性抗原（PSA）基因的引物，通过逆转录聚合酶链反应（RT-PCR）来检测盆腔淋巴结转移。对24例前列腺癌患者进行了盆腔淋巴结细针穿刺活检（FNAB）。每个穿刺样本（0.05 - 0.1毫升）分为两部分；一部分用于RNA提取和RT-PCR以检测PSA mRNA片段，另一部分涂片于载玻片上进行常规细胞学检查。在11个FNAB样本中通过RT-PCR检测到了PSA基因，这些样本不仅包括所有6例细胞学阳性和2例细胞学III级病例，还包括16例细胞学阴性病例中的3例。在20例膀胱癌患者的任何FNAB样本中，通过RT-PCR均未检测到PSA基因。因此，在FNAB样本中进行RT-PCR检测PSA基因可能作为一种检测前列腺癌早期淋巴结转移的新诊断技术，以及作为前列腺癌细胞学诊断的一种辅助工具。
