Grever W E, Chiu F C, Tricoche M, Rashbaum W K, Weidenheim K M, Lyman W D
Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
J Comp Neurol. 1996 Dec 9;376(2):306-14. doi: 10.1002/(SICI)1096-9861(19961209)376:2<306::AID-CNE11>3.0.CO;2-J.
The amount of myelin basic protein (MBP) was quantified in human fetal spinal cords from 12 to 24 gestational weeks (GW). MBP expression was determined by Northern blot, quantitative immunoblot, and immunocytochemistry. The development of compact myelin was analyzed by electron microscopy. Thirty-eight human fetal spinal cords were obtained after elective termination of intrauterine pregnancies from healthy women. Northern blot analysis showed a 15.8-fold increase in MBP mRNA between 12 and 18 GW. From 18 to 24 GW, MBP mRNA increased by 2.2-fold. The mRNA data paralleled immunoblot results that showed a 90.5-fold increase in MBP (0.147 ng/mg to 13.3 ng/mg tissue) between 12 and 18 GW and an approximately 11.5-fold increase between 18 and 24 GW (13.3 ng/mg to 154 ng/mg tissue). Immunocytochemical analysis also showed increased staining for MBP with advancing gestational age. At 12 GW, MBP immunoreactivity was observed in all three spinal cord funiculi. By 18 GW, MBP was expressed throughout the spinal cord white matter with the exception of the lateral corticospinal tracts and in the rostral levels of the fasciculus gracilis. With respect to myelin, at 12 GW, rare, noncompacted myelin lamellae were observed by electron microscopy. By 18 GW, discrete areas of compact myelin were observed in areas that showed MBP immunoreactivity, and at 24 GW, compact myelin was prominent throughout the white matter of the spinal cord. This study demonstrates a quantitative increase in MBP expression that is associated with myelin formation during the second trimester of human gestation. This information may provide normative data that can aid in the diagnosis of myelin disorders of the preterm, neonatal, and pediatric spinal cord.
对妊娠12至24周（GW）的人胎儿脊髓中髓鞘碱性蛋白（MBP）的含量进行了定量分析。通过Northern印迹法、定量免疫印迹法和免疫细胞化学法测定MBP的表达。通过电子显微镜分析紧密髓鞘的发育情况。从健康女性的宫内妊娠选择性终止后获取了38条人胎儿脊髓。Northern印迹分析显示，在12至18 GW之间，MBP mRNA增加了15.8倍。从18至24 GW，MBP mRNA增加了2.2倍。mRNA数据与免疫印迹结果平行，免疫印迹结果显示，在12至18 GW之间，MBP增加了90.5倍（从0.147 ng/mg组织增加到13.3 ng/mg组织），在18至24 GW之间增加了约11.5倍（从13.3 ng/mg组织增加到154 ng/mg组织）。免疫细胞化学分析也显示，随着胎龄的增加，MBP染色增强。在12 GW时，在所有三个脊髓索中均观察到MBP免疫反应性。到18 GW时，除皮质脊髓侧束和薄束的头端水平外，MBP在整个脊髓白质中均有表达。关于髓鞘，在12 GW时通过电子显微镜观察到罕见的、未紧密排列的髓鞘板层。到18 GW时，在显示MBP免疫反应性的区域观察到离散的紧密髓鞘区域，在24 GW时，紧密髓鞘在脊髓白质中很突出。这项研究表明，在人类妊娠中期，MBP表达的定量增加与髓鞘形成有关。这些信息可能提供有助于诊断早产、新生儿和小儿脊髓髓鞘疾病的标准数据。
