Weidenheim K M, Epshteyn I, Rashbaum W K, Lyman W D
Department of Pathology (Neuropathology), Albert Einstein College of Medicine, Bronx, New York 10461.
J Neurocytol. 1993 Jul;22(7):507-16. doi: 10.1007/BF01189039.
The temporal and spatial expression of myelin basic protein in the first and second trimester human foetal spinal cord and brainstem from 9 to 20 gestational weeks was determined by immunocytochemistry in sections of cervical, thoracic and lumbosacral levels from 41 human foetal spinal cords and ten brainstems. Myelin basic protein-positive oligodendrocytes were observed peripheral to the ependyma at 9-10 gestational weeks. Oligodendrocytes expressing myelin basic protein were seen at 10-12 gestational weeks in the anterior and lateral funiculi. Myelin basic protein was detected later in the posterior funiculi than in the anterolateral white matter and most spinal cord tracts could not be identified by means of variation in myelin basic protein expression. Myelin basic protein was found in the midline of the brainstem at ten gestational weeks and spread laterally during the second trimester. We conclude that in the human foetal spinal cord, myelin basic protein is present by 10 gestational weeks in the anterolateral cervical spinal cord and midline of the brainstem. It is expressed in a rostral-to-caudal and anterolateral-to-posterior manner in most tracts of the spinal cord. However, an exception to these findings is that the fasciculus gracilis, upon developing into a defined region, had more myelin basic protein-positive cells at the lumbar level than in more rostral regions. Definition of the kinetics of myelin basic protein expression in the normal human foetal spinal cord provides a baseline for study of aberrant myelination and demyelination.
通过免疫细胞化学方法，对41例人类胎儿脊髓和10例脑干颈段、胸段和腰骶段水平的切片进行研究，确定了妊娠9至20周时人胎儿脊髓和脑干中髓鞘碱性蛋白的时空表达。在妊娠9 - 10周时，在室管膜外周观察到髓鞘碱性蛋白阳性的少突胶质细胞。在妊娠10 - 12周时，在前索和外侧索中可见表达髓鞘碱性蛋白的少突胶质细胞。髓鞘碱性蛋白在后索中的出现晚于前外侧白质，且大多数脊髓束无法通过髓鞘碱性蛋白表达的变化来识别。在妊娠10周时，在脑干中线发现髓鞘碱性蛋白，并在妊娠中期向外侧扩散。我们得出结论，在人类胎儿脊髓中，妊娠10周时在前外侧颈脊髓和脑干中线存在髓鞘碱性蛋白。它在脊髓的大多数束中以从吻侧到尾侧、从前外侧到后外侧的方式表达。然而，这些发现的一个例外是，薄束在发育成一个明确的区域后，在腰段比在更高的头端区域有更多的髓鞘碱性蛋白阳性细胞。确定正常人胎儿脊髓中髓鞘碱性蛋白表达的动力学为研究异常髓鞘形成和脱髓鞘提供了一个基线。
