Tanaka S, Mito T, Takashima S
Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, Tokyo, Japan.
Early Hum Dev. 1995 Mar 17;41(1):49-59. doi: 10.1016/0378-3782(94)01608-r.
The early progress of myelination was studied, by means of myelin basic protein (MBP) immunohistochemistry and luxol-fast-blue (LFB) staining, in the spinal cord, spinal nerve roots and brainstem of 66 fetuses and neonates. The degree of myelination was classified from 1 (slight) to 4 (mature). MBP immunoreactivity exhibited slight LFB positivity. Myelination first occurred in the medial longitudinal fasciculus at 20 weeks of age, reaching degree 4 at 34 weeks, but began at 23-24 weeks in the other sites. Myelination of spinal nerve roots progressed with gestation and attained degree 4 at 35-36 weeks. The cuneate fasciculus also reached degree 4 at 34-36 weeks, but corticospinal tracts and solitary tracts, which exhibited long myelinating phases, were slow and incomplete at 40 weeks. This normal development of MBP and LFB myelination can be used for the assessment of delayed myelination in fetal and neonatal diseases.
通过髓鞘碱性蛋白（MBP）免疫组织化学和卢戈氏坚牢蓝（LFB）染色，研究了66例胎儿和新生儿脊髓、脊神经根及脑干的早期髓鞘形成进展。髓鞘形成程度分为1级（轻度）至4级（成熟）。MBP免疫反应性表现为轻度LFB阳性。髓鞘形成于20周龄时首先出现在内侧纵束，34周时达到4级，但在其他部位于23 - 24周开始。脊神经根的髓鞘形成随孕周进展，35 - 36周时达到4级。楔束在34 - 36周时也达到4级，但皮质脊髓束和孤束髓鞘形成期较长，在40周时缓慢且不完全。MBP和LFB髓鞘形成的这种正常发育可用于评估胎儿和新生儿疾病中的髓鞘形成延迟情况。
