Geinisman Y, Bondareff W, Telser A
Mech Ageing Dev. 1977 Sep-Oct;6(5):363-78. doi: 10.1016/0047-6374(77)90038-0.
At various time intervals (10, 15, 20, 25, 30 min) after injection of 3H-fucose into the medial septal nucleus of young adult (3 months old) and senescent (25 months old) Fischer-344 rats, the specific activities of trichloroacetic acid-phosphotungstic acid (TCA-PTA) soluble and insoluble fractions were determined in the medial area of the septum and in three successive rostro-caudal sections of the hippocampal formation containing mainly the dentate gyrus, but also its hilus with fields CA4 and CA3c of the hippocampus. The rate of 3H-fucose incorporation into glycoproteins of the septum did not differ in young adult and senescent rats. Part of the TCA-PTA soluble and insoluble radioactive material was transported through the septo-hippocampal pathway to the dentate gyrus. This transport was inhibited by the injection of colchicine into the septum prior to 3H-fucose injection and was completely blocked by electrolytic lesion of the medial septal nucleus. The arrival time and the amount of the TCA-PTA soluble radioactive material transported to the dentate gyrus did not differ in young adult and senescent rats. However, the TCA-PTA insoluble labelled glycoprotein was transported to the dentate gyrus in a significantly smaller amount and during a longer period of time in the senescent animals. This age-related change may reflect a reduction in amount and/or in rate of axonal transport of glycoproteins in the septo-hippocampal pathway of senescent rats.
在向年轻成年（3个月大）和衰老（25个月大）的Fischer-344大鼠内侧隔核注射3H-岩藻糖后的不同时间间隔（10、15、20、25、30分钟），测定隔区内侧以及海马结构三个连续的 rostro-caudal 切片中三氯乙酸-磷钨酸（TCA-PTA）可溶性和不溶性部分的比活性，这些切片主要包含齿状回，也包括其海马门以及海马的CA4和CA3c区。3H-岩藻糖掺入年轻成年大鼠和衰老大鼠隔区糖蛋白的速率没有差异。部分TCA-PTA可溶性和不溶性放射性物质通过隔-海马通路转运至齿状回。在注射3H-岩藻糖之前向隔区注射秋水仙碱可抑制这种转运，而内侧隔核的电解损伤可完全阻断这种转运。转运至齿状回的TCA-PTA可溶性放射性物质的到达时间和量在年轻成年大鼠和衰老大鼠中没有差异。然而，衰老动物中TCA-PTA不溶性标记糖蛋白转运至齿状回的量明显较少，且转运时间较长。这种与年龄相关的变化可能反映了衰老大鼠隔-海马通路中糖蛋白轴突运输量和/或运输速率的降低。
