Kato N, Kato H, Watanabe K, Ueno K
Institute of Anaerobic Bacteriology, Gifu University School of Medicine, Japan.
Clin Infect Dis. 1996 Dec;23 Suppl 1:S83-6. doi: 10.1093/clinids/23.supplement_1.s83.
Polymerase chain reaction (PCR) assay was compared with cell culture assay performed with use of HT29/C1 (human colonic epithelial) cells for identifying strains of enterotoxin-producing Bacteroides fragilis (ETBF) isolated from extraintestinal specimens. A total of 188 unselected strains obtained over 2 years at a central clinical laboratory in Tokyo were tested. Overall, 35 strains (18.6%) were positive by cell culture and PCR assay, 152 strains were negative by both assays, and 1 strain was negative by cell culture assay but positive by the PCR assay; the same results were obtained in repeated assays. Among 64 strains from blood, 18 (28.1%) were ETBF, a rate that was significantly higher (P < .05) than the 17 ETBF (13.7%) among 124 strains from other sites. These results suggest that PCR assay is a simple and reliable tool for detecting ETBF and that enterotoxin may be a virulence factor in bacteremia caused by B. fragilis.
将聚合酶链反应（PCR）检测法与使用HT29/C1（人结肠上皮）细胞进行的细胞培养检测法相比较，以鉴定从肠外标本中分离出的产肠毒素脆弱拟杆菌（ETBF）菌株。对东京一家中心临床实验室在两年内获得的总共188株未经筛选的菌株进行了检测。总体而言，35株菌株（18.6%）通过细胞培养和PCR检测呈阳性，152株菌株两种检测均为阴性，1株菌株细胞培养检测为阴性但PCR检测为阳性；重复检测得到相同结果。在64株血液来源的菌株中，18株（28.1%）为ETBF，这一比例显著高于其他部位来源的124株菌株中的17株ETBF（13.7%）（P < .05）。这些结果表明，PCR检测法是检测ETBF的一种简单且可靠的工具，并且肠毒素可能是脆弱拟杆菌引起菌血症的一种毒力因子。
