采用脉冲安培检测的高pH值阴离子交换色谱法及人乳寡糖的摩尔响应因子
High-pH anion-exchange chromatography with pulsed amperometric detection and molar response factors of human milk oligosaccharides.
作者信息
Kunz C, Rudloff S, Hintelmann A, Pohlentz G, Egge H
机构信息
Research Institute for child Nutrition, Dortmund, Germany.
出版信息
J Chromatogr B Biomed Appl. 1996 Oct 25;685(2):211-21. doi: 10.1016/s0378-4347(96)00181-8.
A method is described to separate and characterize neutral and acidic lactose-derived oligosaccharides without prior derivatization or reduction by high-pH anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). This method has been applied to human milk oligosaccharides from donors with different blood group specificity (A, Le(a) and A, Le(b). Neutral and acidic components were separated from each other by anion-exchange chromatography. A distinct separation of individual components was obtained by size-exclusion chromatography on Fractogel TSK HW 50S (acidic oligosaccharides) or Fractogel TSK HW 40S (neutral oligosaccharides containing up to 6 monomers) and Bio-Gel P-4 size exclusion (neutral oligosaccharides containing more than 6 monomers). Furthermore the moral response factors after HPAEC-PAD have been determined for 28 components.
描述了一种通过高pH值阴离子交换色谱法和脉冲安培检测法（HPAEC-PAD）分离和表征中性和酸性乳糖衍生寡糖的方法，无需事先进行衍生化或还原。该方法已应用于来自具有不同血型特异性（A、Le(a)和A、Le(b)）供体的人乳寡糖。通过阴离子交换色谱法将中性和酸性成分彼此分离。通过在Fractogel TSK HW 50S（酸性寡糖）或Fractogel TSK HW 40S（含多达6个单体的中性寡糖）上进行尺寸排阻色谱法以及Bio-Gel P-4尺寸排阻（含超过6个单体的中性寡糖），实现了各个成分的明显分离。此外，还测定了28种成分在HPAEC-PAD后的摩尔响应因子。
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