土壤中二甲基硅二醇的生物降解
Biodegradation of dimethylsilanediol in soils.
作者信息
Sabourin C L, Carpenter J C, Leib T K, Spivack J L
机构信息
Environmental Laboratory, General Electric Corporate Research and Development, Schenectady, New York 12301, USA.
出版信息
Appl Environ Microbiol. 1996 Dec;62(12):4352-60. doi: 10.1128/aem.62.12.4352-4360.1996.
The biodegradation potential of [14C]dimethylsilanediol, the monomer unit of polydimethylsiloxane, in soils was investigated. Dimethylsilanediol was found to be biodegraded in all of the tested soils, as monitored by the production of 14CO2. When 2-propanol was added to the soil as a carbon source in addition to [14C]dimethylsilanediol, the production of 14CO2 increased. A method for the selection of primary substrates that support cometabolic degradation of a target compound was developed. By this method, the activity observed in the soils was successfully transferred to liquid culture. A fungus, Fusarium oxysporum Schlechtendahl, and a bacterium, an Arthrobacter species, were isolated from two different soils, and both microorganisms were able to cometabolize [14C]dimethylsilanediol to 14CO2 in liquid culture. In addition, the Arthrobacter sp. that was isolated grew on dimethylsulfone, and we believe that this is the first reported instance of a microorganism using dimethylsulfone as its primary carbon source. Previous evidence has shown that polydimethylsiloxane is hydrolyzed in soil to the monomer, dimethylsilanediol. Now, biodegradation of dimethylsilanediol in soil has been demonstrated.
研究了聚二甲基硅氧烷的单体单元[14C]二甲基硅二醇在土壤中的生物降解潜力。通过监测14CO2的产生发现，在所有测试土壤中，二甲基硅二醇都能被生物降解。当除了[14C]二甲基硅二醇之外，向土壤中添加2-丙醇作为碳源时，14CO2的产生量增加。开发了一种选择支持目标化合物共代谢降解的主要底物的方法。通过这种方法，在土壤中观察到的活性成功转移到了液体培养物中。从两种不同的土壤中分离出一种真菌，尖孢镰刀菌（Fusarium oxysporum Schlechtendahl）和一种细菌，节杆菌属（Arthrobacter species），并且这两种微生物在液体培养中都能够将[14C]二甲基硅二醇共代谢为14CO2。此外，分离出的节杆菌（Arthrobacter sp.）能够在二甲基砜上生长，并且我们认为这是首次报道微生物将二甲基砜用作其主要碳源的实例。先前的证据表明，聚二甲基硅氧烷在土壤中水解为单体二甲基硅二醇。现在，已证明二甲基硅二醇在土壤中的生物降解。
Zotero 插件