Zhao H R, Smith J B, Jiang X Y, Abraham E C
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912-2100, USA.
Biochem Biophys Res Commun. 1996 Dec 4;229(1):128-33. doi: 10.1006/bbrc.1996.1768.
We determined the sites of glycation of bovine beta B2-crystallin by glucose and fructose. After incubation with glucose or fructose, glycated tryptic peptides were purified by affinity chromatography/reverse-phase HPLC and identified by electrospray ionization mass spectrometry (ESIMS). The results gave evidence of glycation at lysine 10, 75, 100, 107, 120, 139, 167 and 171 by both glucose and fructose, while glycated lysine 119 and 47 or 67 were detected only after fructosylation. We conclude that glucose and fructose have similar glycation specificity.
我们确定了葡萄糖和果糖对牛βB2-晶体蛋白的糖基化位点。在与葡萄糖或果糖孵育后，通过亲和色谱/反相高效液相色谱法纯化糖化胰蛋白酶肽，并通过电喷雾电离质谱法（ESIMS）进行鉴定。结果表明，葡萄糖和果糖均可使赖氨酸10、75、100、107、120、139、167和171发生糖基化，而仅在果糖基化后才检测到糖化赖氨酸119以及47或67。我们得出结论，葡萄糖和果糖具有相似的糖基化特异性。
