Barres BA, Burne JF, Holtmann B, Thoenen H, Sendtner M, Raff MC
Department of Neurobiology, Fairchild Science Building, Stanford University School of Medicine, Stanford, California, 94305-5401
Mol Cell Neurosci. 1996 Aug;8(2/3):146-56. doi: 10.1006/mcne.1996.0053.
Although ciliary neurotrophic factor (CNTF) is a potent survival factor for many types of neurons and glial cells in vitro, there is currently no evidence that it participates in normal development. Here we show that CNTF greatly enhances the rate of oligodendrocyte generation. Proliferation of oligodendrocyte precursor cells purified from rodent optic nerves and cultured in platelet-derived growth factor-containing medium is significantly increased by CNTF. Similarly, the number of proliferating oligodendrocyte precursor cells in developing optic nerves of transgenic mice lacking CNTF is decreased by up to threefold and the number of oligodendrocytes is transiently decreased; proliferation is restored to normal by the delivery of exogenous CNTF into the developing optic nerve. Both oligodendrocyte number and myelination ultimately attain wild-type values in CNTF-deficient adult mice, indicating that CNTF is not necessary for either oligodendrocyte differentiation or myelination, although it normally accelerates oligodendrocyte development by enhancing the proliferation of oligodendrocyte precursor cells.
尽管睫状神经营养因子（CNTF）在体外对多种类型的神经元和神经胶质细胞是一种有效的存活因子，但目前尚无证据表明它参与正常发育。在此我们表明，CNTF能极大地提高少突胶质细胞的生成速率。从啮齿动物视神经中纯化并在含血小板衍生生长因子的培养基中培养的少突胶质细胞前体细胞的增殖，被CNTF显著增加。同样，在缺乏CNTF的转基因小鼠发育中的视神经中，增殖的少突胶质细胞前体细胞数量减少了多达三倍，少突胶质细胞数量也短暂减少；通过将外源性CNTF递送至发育中的视神经，增殖恢复正常。在缺乏CNTF的成年小鼠中，少突胶质细胞数量和髓鞘形成最终都达到野生型值，这表明CNTF对于少突胶质细胞分化或髓鞘形成并非必需，尽管它通常通过增强少突胶质细胞前体细胞的增殖来加速少突胶质细胞发育。
