Sato K, Tanaka T, Okamoto H, Miyakawa Y, Mayumi M
Japanese Red Cross Saitama Blood Center, Saitama-Ken, 338, Japan.
Biochem Biophys Res Commun. 1996 Dec 24;229(3):719-25. doi: 10.1006/bbrc.1996.1871.
Buoyant density of a recently discovered putative non-A to E hepatitis virus designated hepatitis G virus (HGV) was estimated in plasma or serum samples from three symptom-free carriers and two hepatitis patients. HGV RNA was detected by reverse-transcription polymerase chain reaction in sucrose density fractions with a low density at 1.05-1.10 g/cm3, and the density shifted to 1.23-1.26 g/cm3 after a treatment of peak fractions with Tween 80. Fractionated HGV was precipitated with antibodies to apolipoproteins, but not at all with antibodies to IgG; it was retained by affinity columns of lectins. These results indicate that the circulating HGV would be covered with lipoproteins of the host and has sugar moieties on the surface. The association of HGV with lipids would be responsible for the observed low density and prevent the binding with antibodies for viral persistence.
在三名无症状携带者和两名肝炎患者的血浆或血清样本中，对最近发现的一种假定的非甲至戊型肝炎病毒——庚型肝炎病毒（HGV）的浮力密度进行了估算。通过逆转录聚合酶链反应在蔗糖密度梯度分馏物中检测到HGV RNA，其低密度在1.05 - 1.10 g/cm³，在用吐温80处理峰值分馏物后，密度转移至1.23 - 1.26 g/cm³。分级分离的HGV可被载脂蛋白抗体沉淀，但完全不能被IgG抗体沉淀；它能被凝集素亲和柱保留。这些结果表明，循环中的HGV会被宿主的脂蛋白覆盖，且表面具有糖基部分。HGV与脂质的结合可能是观察到的低密度的原因，并可防止其与抗体结合以实现病毒持续存在。
