Vitelli R, Chiariello M, Lattero D, Bruni C B, Bucci C
Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano", Università degli Studi di Napoli "Federico II,", Via S. Pansini 5, Naples, Italy.
Biochem Biophys Res Commun. 1996 Dec 24;229(3):887-90. doi: 10.1006/bbrc.1996.1897.
Rab7 is a small GTP-ase localized on late endosomes, which regulates late endocytic membrane traffic in mammalian cells. Moreover it has been shown that this protein has a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. We report here for the first time the isolation of a cDNA encoding human Rab7 from a placenta cDNA library. The open reading frame for human Rab7 encodes a protein of 207 amino acids which exhibits high homology with the mouse, rat, and dog counterparts. Northern blot analysis of total RNAs isolated from different cell lines with a cDNA probe containing the entire open reading frame revealed two mRNA transcripts of 2.5 and 1.8 kilobases. The isolation of human Rab7 cDNA will allow further characterization of its function in normal and pathological states.
Rab7是一种定位于晚期内体的小GTP酶，它调节哺乳动物细胞中的晚期内吞膜运输。此外，已经表明该蛋白在幽门螺杆菌细胞毒素VacA诱导的细胞空泡化中起重要作用。我们在此首次报道从胎盘cDNA文库中分离出编码人Rab7的cDNA。人Rab7的开放阅读框编码一种207个氨基酸的蛋白质，它与小鼠、大鼠和犬的对应物具有高度同源性。用包含整个开放阅读框的cDNA探针，对从不同细胞系分离的总RNA进行Northern印迹分析，发现了2.5和1.8千碱基的两种mRNA转录本。人Rab7 cDNA的分离将有助于进一步表征其在正常和病理状态下的功能。
