Dunstan C A, Salafranca M N, Adhikari S, Xia Y, Feng L, Harrison J K
Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville, Florida 32610-0267, USA.
J Biol Chem. 1996 Dec 20;271(51):32770-6. doi: 10.1074/jbc.271.51.32770.
The genes encoding two functional human interleukin-8 (IL-8) receptors have been identified by molecular cloning techniques and they are members of the rhodopsin G-protein coupled receptor (GCR) superfamily. We report the molecular cloning of two rat GCR genes (rat CXCR1-like and rat CXCR2) whose conceptualized amino acid sequences are approximately 70% identical to the human IL-8 A and B receptor subtypes. The murine GRO-like peptide, macrophage inflammatory peptide-2 (MIP-2), elevates intracellular calcium levels in HEK293 cells expressing the rat CXCR2 receptor. Southern blot analysis of restriction-digested rodent and human genomic DNAs indicate that rat CXCR1-like and CXCR2 are: 1) each single copy genes in the rat genome; 2) most closely related to the human IL-8 receptor genes; and 3) orthologous to two previously identified murine genes. CXCR2 mRNA is detected in adult rat lung, spleen, and neutrophils. CXCR1-like mRNA can be detected in adult rat lung, native rat macrophages, and a rat alveolar macrophage cell line (NR8383). These data identify the rat orthologs of the human IL-8 receptors, and describe cellular and tissue targets of rat C-X-C chemokine peptides.
通过分子克隆技术已鉴定出编码两种功能性人类白细胞介素8（IL-8）受体的基因，它们是视紫红质G蛋白偶联受体（GCR）超家族的成员。我们报告了两个大鼠GCR基因（大鼠CXCR1样基因和大鼠CXCR2）的分子克隆，其概念化的氨基酸序列与人类IL-8 A和B受体亚型约70%相同。小鼠GRO样肽、巨噬细胞炎性蛋白-2（MIP-2）可提高表达大鼠CXCR2受体的HEK293细胞内的钙水平。对经限制性消化的啮齿动物和人类基因组DNA进行的Southern印迹分析表明，大鼠CXCR1样基因和CXCR2是：1）大鼠基因组中的单拷贝基因；2）与人类IL-8受体基因关系最密切；3）与两个先前鉴定的小鼠基因直系同源。在成年大鼠肺、脾和中性粒细胞中检测到CXCR2 mRNA。在成年大鼠肺、原代大鼠巨噬细胞和大鼠肺泡巨噬细胞系（NR8383）中可检测到CXCR1样mRNA。这些数据确定了人类IL-8受体的大鼠直系同源物，并描述了大鼠C-X-C趋化因子肽的细胞和组织靶点。
