Centre for Inflammation Research, University of Edinburgh Centre for Inflammation Research, Edinburgh, United Kingdom.
Simons Initiative for the Developing Brain, University of Edinburgh, Edinburgh, United Kingdom.
Front Immunol. 2021 Feb 1;11:594594. doi: 10.3389/fimmu.2020.594594. eCollection 2020.
The laboratory rat is widely used as a model for human diseases. Many of these diseases involve monocytes and tissue macrophages in different states of activation. Whilst methods for differentiation of mouse macrophages from embryonic stem cells (ESC) and bone marrow (BM) are well established, these are lacking for the rat. The gene expression profiles of rat macrophages have also not been characterised to the same extent as mouse. We have established the methodology for production of rat ESC-derived macrophages and compared their gene expression profiles to macrophages obtained from the lung and peritoneal cavity and those differentiated from BM and blood monocytes. We determined the gene signature of Kupffer cells in the liver using rats deficient in macrophage colony stimulating factor receptor (CSF1R). We also examined the response of BM-derived macrophages to lipopolysaccharide (LPS). The results indicate that many, but not all, tissue-specific adaptations observed in mice are conserved in the rat. Importantly, we show that unlike mice, rat macrophages express the CSF1R ligand, colony stimulating factor 1 (CSF1).
实验大鼠被广泛用作人类疾病的模型。许多这些疾病涉及单核细胞和组织巨噬细胞处于不同的激活状态。虽然已经建立了从胚胎干细胞（ESC）和骨髓（BM）分化小鼠巨噬细胞的方法，但这些方法在大鼠中却缺乏。大鼠巨噬细胞的基因表达谱也没有像小鼠那样得到充分的描述。我们已经建立了生产大鼠 ESC 衍生的巨噬细胞的方法，并将其基因表达谱与从肺和腹腔获得的巨噬细胞以及从 BM 和血液单核细胞分化而来的巨噬细胞进行了比较。我们使用缺乏巨噬细胞集落刺激因子受体（CSF1R）的大鼠确定了肝脏中枯否细胞的基因特征。我们还研究了 BM 衍生的巨噬细胞对脂多糖（LPS）的反应。结果表明，许多在小鼠中观察到的组织特异性适应在大鼠中得到了保守，但并非所有适应都得到了保守。重要的是，我们表明与小鼠不同，大鼠巨噬细胞表达 CSF1R 配体，集落刺激因子 1（CSF1）。
