柑橘螺原体和蜜蜂螺原体recA基因区域的特征分析。
Characterization of the recA gene regions of Spiroplasma citri and Spiroplasma melliferum.
作者信息
Marais A, Bove J M, Renaudin J
机构信息
Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique and Université de Bordeaux II, Villenave d'Ornon, France.
出版信息
J Bacteriol. 1996 Dec;178(23):7003-9. doi: 10.1128/jb.178.23.7003-7009.1996.
Abstract
In previous studies (A. Marais, J. M. Bove, and J. Renaudin, J. Bacteriol. 178:862-870, 1996), we have shown that the recA gene of Spiroplasma citri R8A2 was restricted to the first 390 nucleotides of the N-terminal part. PCR amplification and sequencing studies of five additional strains of S. citri have revealed that these strains had the same organization at the recA region as the R8A2 strain. In contrast to S. citri, Spiroplasma melliferum was found to contain a full-length recA gene. However, in all five S. melliferum strains tested, a TAA stop codon was found within the N-terminal region of the recA reading frame. Our results suggest that S. melliferum, as well as S. citri, is RecA deficient. In agreement with the recA mutant genotype of S. citri and S. melliferum, we have shown that these organisms are highly sensitive to UV irradiation.
摘要
在之前的研究中（A. 马雷、J. M. 博韦和J. 勒诺丹，《细菌学杂志》178:862 - 870，1996年），我们已经表明，柑橘螺原体R8A2的recA基因局限于N端部分的前390个核苷酸。对另外五株柑橘螺原体菌株的PCR扩增和测序研究表明，这些菌株在recA区域的结构与R8A2菌株相同。与柑橘螺原体不同，发现蜜蜂螺原体含有一个全长recA基因。然而，在所测试的所有五株蜜蜂螺原体菌株中，在recA阅读框的N端区域内发现了一个TAA终止密码子。我们的结果表明，蜜蜂螺原体以及柑橘螺原体都缺乏RecA。与柑橘螺原体和蜜蜂螺原体的recA突变基因型一致，我们已经表明这些生物体对紫外线照射高度敏感。
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